Reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays

Louis A. Magnarelli, Jacob W. Ijdo, J. Stephen Dumler, Robert Heimer, Erol Fikrig

Research output: Contribution to journalArticle

Abstract

Sera from 35 patients diagnosed with human granulocytic ehrlichiosis in Connecticut were tested by indirect IFA staining methods with 5 strains of Ehrlichia equi or the human granulocytic ehrlichiosis agent to assess the suitability of different strains in laboratory analyses. Antigens included horse-derived infected neutrophils (MRK and BDS strains) and human isolates cultured in human promyelocytic leukemia cells (NCH-1, RCH, and Webster). Of 35 sera, 23 (65.7%) reacted to all 5 strains. Seropositivity was highest (97.1%) in assays that contained the MRK strain from California and lowest (71.4%) in tests with the NCH-1 strain from Nantucket, Massachusetts. In parallel testing of 32 sera with the NCH-1 strain by indirect IFA and Western blot analyses, results were concordant for 30 samples (93.8%). All strains of ehrlichiae can be used in IFA analyses for antibody detection, but assay sensitivity varied with the strain used.

Original languageEnglish (US)
Pages (from-to)1835-1838
Number of pages4
JournalJournal of Infectious Diseases
Volume178
Issue number6
DOIs
StatePublished - 1998
Externally publishedYes

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Ehrlichia
Ehrlichiosis
Serum
Anaplasma phagocytophilum
Horses
Leukemia
Neutrophils
Western Blotting
Staining and Labeling
Antigens
Antibodies

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays. / Magnarelli, Louis A.; Ijdo, Jacob W.; Dumler, J. Stephen; Heimer, Robert; Fikrig, Erol.

In: Journal of Infectious Diseases, Vol. 178, No. 6, 1998, p. 1835-1838.

Research output: Contribution to journalArticle

Magnarelli, Louis A. ; Ijdo, Jacob W. ; Dumler, J. Stephen ; Heimer, Robert ; Fikrig, Erol. / Reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 6. pp. 1835-1838.
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