Reactive oxygen species are critical for the growth and differentiation of medullary thyroid carcinoma cells

H. C. Ha, A. Thiagalingam, B. D. Nelkin, Jr Casero

Research output: Contribution to journalArticle

Abstract

Reactive oxygen species have recently been demonstrated to play a role in numerous cellular signal transduction pathways. Here we investigate the involvement of H2O2 in Raf-l-mediated differentiation in the human medullary thyroid carcinoma (MTC) cell line TT:ΔRaf-1:ER. Catalase, but not Cu/Zn superoxide dismutase, completely inhibited Raf-1-induced differentiation of β-estradiol-treated TT:ΔRaf-1:ER. In addition, catalase treatment down-regulated RET expression at both the mRNA and protein levels and induced apoptosis in the parental TT cell line and uninduced TT:ΔRaf-1:ER human MTC cells. These results implicate H2O2 as a downstream mediator of c-Raf-1-induced differentiation and as a survival factor in MTC cells.

Original languageEnglish (US)
Pages (from-to)3783-3787
Number of pages5
JournalClinical Cancer Research
Volume6
Issue number9
StatePublished - Sep 26 2000

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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