Re-classification of Drosophila melanogaster trichoid and intermediate sensilla using fluorescence-guided single sensillum recording

Chun Chieh Lin, Christopher J Potter

Research output: Contribution to journalArticle

Abstract

Drosophila olfactory receptor neurons are found within specialized sensory hairs on antenna and maxillary palps. The linking of odorant-induced responses to olfactory neuron activities is often accomplished via Single Sensillum Recordings (SSR), in which an electrode inserted into a single sensory hair records the neuronal activities of all the neurons housed in that sensillum. The identification of the recorded sensillum requires matching the neuronal responses with known odor-response profiles. To record from specific sensilla, or to systematically screen all sensillar types, requires repetitive and semi-random SSR experiments. Here, we validate an approach in which the GAL4/UAS binary expression system is used for targeting specific sensilla for recordings. We take advantage of available OrX-Gal4 lines, in combination with recently generated strong membrane targeted GFP reporters, to guide electrophysiological recordings to GFP-labeled sensilla. We validate a full set of reagents that can be used to rapidly screen the odor-response profiles of all basiconic, intermediate, and trichoid sensilla. Fluorescence-guided SSR further revealed that two antennal trichoid sensilla types should be re-classified as intermediate sensilla. This approach provides a simple and practical addition to a proven method for investigating olfactory neurons, and can be extended by the addition of UAS-geneX effectors for gain-of-function or loss-offunction studies.

Original languageEnglish (US)
Article numbere0139675
JournalPLoS One
Volume10
Issue number10
DOIs
StatePublished - Oct 2 2015

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Sensilla
sensilla
taxonomic revisions
Drosophila melanogaster
Neurons
Fluorescence
fluorescence
Odors
Odorant Receptors
neurons
Antennas
Membranes
hairs
Electrodes
odors
Olfactory Receptor Neurons
olfactory receptors
palps
Experiments
odor compounds

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Re-classification of Drosophila melanogaster trichoid and intermediate sensilla using fluorescence-guided single sensillum recording. / Lin, Chun Chieh; Potter, Christopher J.

In: PLoS One, Vol. 10, No. 10, e0139675, 02.10.2015.

Research output: Contribution to journalArticle

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