Rax-CreERT2 knock-in mice: A tool for selective and conditional gene deletion in progenitor cells and radial glia of the retina and hypothalamus

Thomas Pak, Soo Yeon Yoo, Ana M. Miranda-Angulo, Hong Wang, Seth Blackshaw

Research output: Contribution to journalArticle

Abstract

To study gene function in neural progenitors and radial glia of the retina and hypothalamus, we developed a Rax-CreERT2 mouse line in which a tamoxifen-inducible Cre recombinase is inserted into the endogenous Rax locus. By crossing Rax-CreERT2 with the Cre-dependent Ai9 reporter line, we demonstrate that tamoxifen-induced Cre activity recapitulates the endogenous Rax mRNA expression pattern. During embryonic development, Cre recombinase activity in Rax-CreERT2 is confined to retinal and hypothalamic progenitor cells, as well as progenitor cells of the posterior pituitary. At postnatal time points, selective Cre recombinase activity is seen in radial glial-like cell types in these organs - specifically Müller glia and tanycytes - as well as pituicytes. We anticipate that this line will prove useful for cell lineage analysis and investigation of gene function in the developing and mature retina, hypothalamus and pituitary.

Original languageEnglish (US)
Article numbere90381
JournalPLoS One
Volume9
Issue number4
DOIs
StatePublished - Apr 3 2014

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tamoxifen
gene deletion
Gene Deletion
hypothalamus
retina
Neuroglia
Hypothalamus
Ependymoglial Cells
stem cells
Retina
Stem Cells
Genes
Tamoxifen
posterior pituitary
mice
neuroglia
embryogenesis
genes
Cell Lineage
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rax-CreERT2 knock-in mice : A tool for selective and conditional gene deletion in progenitor cells and radial glia of the retina and hypothalamus. / Pak, Thomas; Yoo, Soo Yeon; Miranda-Angulo, Ana M.; Wang, Hong; Blackshaw, Seth.

In: PLoS One, Vol. 9, No. 4, e90381, 03.04.2014.

Research output: Contribution to journalArticle

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