Ratiometric measurement and identification of single diffusing molecules

Maxime Dahan, Ashok A. Deniz, Taekjip Ha, Daniel S. Chemla, Peter G. Schultz, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

Abstract

The concept of ratiometric identification and separation of sub-populations of single biomolecules which are labeled with individual fluorophores and are freely diffusing (or flowing) through the laser excitation volume is introduced. The efficacy of the scheme versus the signal and noise level is considered and a general theoretical framework and data analysis schemes are introduced. Experimental results that demonstrate distributions of single molecule fluorescence polarization anisotropy (smFPA) and single pair fluorescence resonance energy transfer (spFRET) in solution are presented. Ultimate resolution for identifying molecular observables such as conformational states, degree of freedom of motion and spectral distribution are discussed.

Original languageEnglish (US)
Pages (from-to)85-106
Number of pages22
JournalChemical Physics
Volume247
Issue number1
DOIs
StatePublished - Aug 1 1999
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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