Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation

M. Mazariegos, C. Veltorazzi, Benjamin H Caballero, C. Field, N. W. Solomons, F. Jahoor

Research output: Contribution to journalArticle

Abstract

It has been s-uggested that chronic immunostimulation, such as occurs in highly contaminated environments, causes a persistent catabolic state, shifting indispensable amino acids toward the synthesis of acute-phase proteins, at the expense of growth. To evaluate this hypothesis, we studied 9 children, aged 3 to 5 y, living in a highly contaminated area (garbage dump of Guatemala City) and who manifested elevations of the cytokine biomarkers (TNFa and/or ILi) A modified approach, to simplify the procedures and develop a protocol for use in young children, and at the community-level was explored, including: 1) the oral (po) administration of isotopes; 2) enrichment of 13CO2 in expired air and a -kctoisocaproic acid (KIC) in plasma for calculations of Leu-oxidation rates; and 3) a total elapsed procedure time of 13CO2-bicarbonate, po challenge-doses of 13CO2-Leu are given every 20 min over an additional 4.5 h. Subjects remain in the fed-state, receiving 75% of recommended daily energy, in divided servings every 20 min. After initiating the challenge doses, isotopic enrichment of expired air is measured every 20 min from 200-280 min. Blood samples were collected at 240, 260, and 280 min. 13CO2 enrichments reached 0.045 - 0.050 APE by 120 min in all cases. Calculated Leu-oxidanon rates ranged from 26.6 - 74,1 umol/kg/h. We conclude that a simplified field approach to measurement of leucine oxidation is at hand.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

immunostimulation (physiological)
Immunization
Air
Garbage
oxidation
Guatemala
air
Oxidation
acute phase proteins
Acute-Phase Proteins
municipal solid waste
Biomarkers
Bicarbonates
dosage
bicarbonates
Leucine
Isotopes
leucine
Oral Administration
mouth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Mazariegos, M., Veltorazzi, C., Caballero, B. H., Field, C., Solomons, N. W., & Jahoor, F. (1996). Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation. FASEB Journal, 10(3).

Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation. / Mazariegos, M.; Veltorazzi, C.; Caballero, Benjamin H; Field, C.; Solomons, N. W.; Jahoor, F.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Mazariegos, M, Veltorazzi, C, Caballero, BH, Field, C, Solomons, NW & Jahoor, F 1996, 'Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation', FASEB Journal, vol. 10, no. 3.
Mazariegos M, Veltorazzi C, Caballero BH, Field C, Solomons NW, Jahoor F. Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation. FASEB Journal. 1996;10(3).
Mazariegos, M. ; Veltorazzi, C. ; Caballero, Benjamin H ; Field, C. ; Solomons, N. W. ; Jahoor, F. / Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
@article{a4433c3390c1468c929eb51b7132fae7,
title = "Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation",
abstract = "It has been s-uggested that chronic immunostimulation, such as occurs in highly contaminated environments, causes a persistent catabolic state, shifting indispensable amino acids toward the synthesis of acute-phase proteins, at the expense of growth. To evaluate this hypothesis, we studied 9 children, aged 3 to 5 y, living in a highly contaminated area (garbage dump of Guatemala City) and who manifested elevations of the cytokine biomarkers (TNFa and/or ILi) A modified approach, to simplify the procedures and develop a protocol for use in young children, and at the community-level was explored, including: 1) the oral (po) administration of isotopes; 2) enrichment of 13CO2 in expired air and a -kctoisocaproic acid (KIC) in plasma for calculations of Leu-oxidation rates; and 3) a total elapsed procedure time of 13CO2-bicarbonate, po challenge-doses of 13CO2-Leu are given every 20 min over an additional 4.5 h. Subjects remain in the fed-state, receiving 75{\%} of recommended daily energy, in divided servings every 20 min. After initiating the challenge doses, isotopic enrichment of expired air is measured every 20 min from 200-280 min. Blood samples were collected at 240, 260, and 280 min. 13CO2 enrichments reached 0.045 - 0.050 APE by 120 min in all cases. Calculated Leu-oxidanon rates ranged from 26.6 - 74,1 umol/kg/h. We conclude that a simplified field approach to measurement of leucine oxidation is at hand.",
author = "M. Mazariegos and C. Veltorazzi and Caballero, {Benjamin H} and C. Field and Solomons, {N. W.} and F. Jahoor",
year = "1996",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Rates of [1-13C]oxidation in guatemalan children with chronic immunostimulation

AU - Mazariegos, M.

AU - Veltorazzi, C.

AU - Caballero, Benjamin H

AU - Field, C.

AU - Solomons, N. W.

AU - Jahoor, F.

PY - 1996

Y1 - 1996

N2 - It has been s-uggested that chronic immunostimulation, such as occurs in highly contaminated environments, causes a persistent catabolic state, shifting indispensable amino acids toward the synthesis of acute-phase proteins, at the expense of growth. To evaluate this hypothesis, we studied 9 children, aged 3 to 5 y, living in a highly contaminated area (garbage dump of Guatemala City) and who manifested elevations of the cytokine biomarkers (TNFa and/or ILi) A modified approach, to simplify the procedures and develop a protocol for use in young children, and at the community-level was explored, including: 1) the oral (po) administration of isotopes; 2) enrichment of 13CO2 in expired air and a -kctoisocaproic acid (KIC) in plasma for calculations of Leu-oxidation rates; and 3) a total elapsed procedure time of 13CO2-bicarbonate, po challenge-doses of 13CO2-Leu are given every 20 min over an additional 4.5 h. Subjects remain in the fed-state, receiving 75% of recommended daily energy, in divided servings every 20 min. After initiating the challenge doses, isotopic enrichment of expired air is measured every 20 min from 200-280 min. Blood samples were collected at 240, 260, and 280 min. 13CO2 enrichments reached 0.045 - 0.050 APE by 120 min in all cases. Calculated Leu-oxidanon rates ranged from 26.6 - 74,1 umol/kg/h. We conclude that a simplified field approach to measurement of leucine oxidation is at hand.

AB - It has been s-uggested that chronic immunostimulation, such as occurs in highly contaminated environments, causes a persistent catabolic state, shifting indispensable amino acids toward the synthesis of acute-phase proteins, at the expense of growth. To evaluate this hypothesis, we studied 9 children, aged 3 to 5 y, living in a highly contaminated area (garbage dump of Guatemala City) and who manifested elevations of the cytokine biomarkers (TNFa and/or ILi) A modified approach, to simplify the procedures and develop a protocol for use in young children, and at the community-level was explored, including: 1) the oral (po) administration of isotopes; 2) enrichment of 13CO2 in expired air and a -kctoisocaproic acid (KIC) in plasma for calculations of Leu-oxidation rates; and 3) a total elapsed procedure time of 13CO2-bicarbonate, po challenge-doses of 13CO2-Leu are given every 20 min over an additional 4.5 h. Subjects remain in the fed-state, receiving 75% of recommended daily energy, in divided servings every 20 min. After initiating the challenge doses, isotopic enrichment of expired air is measured every 20 min from 200-280 min. Blood samples were collected at 240, 260, and 280 min. 13CO2 enrichments reached 0.045 - 0.050 APE by 120 min in all cases. Calculated Leu-oxidanon rates ranged from 26.6 - 74,1 umol/kg/h. We conclude that a simplified field approach to measurement of leucine oxidation is at hand.

UR - http://www.scopus.com/inward/record.url?scp=33749139288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749139288&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749139288

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -