Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV

Henriette Geyer; Jakob Ettinger; Lars Möller; Erik Schmolz; Andreas Nitsche; Wolfram Brune; Sarah Heaggans; Gordon R. Sandford; Gary S. Hayward; Sebastian Voigt

doi:10.1099/vir.0.000109

Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV

Henriette Geyer, Jakob Ettinger, Lars Möller, Erik Schmolz, Andreas Nitsche, Wolfram Brune, Sarah Heaggans, Gordon R. Sandford, Gary S. Hayward, Sebastian Voigt

School of Medicine

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9 Scopus citations

Abstract

The genome of the rat cytomegalovirus (RCMV) English isolate (MuHV-8) differs significantly from the RCMV Maastricht isolate (MuHV-2) and other cytomegaloviruses (CMVs) in its size, base composition and genomic content. Analysis of the RCMV-Berlin isolate, MuHV-8, revealed that the two MuHV-8 isolates are highly similar in genome size and content, indicating that the smaller genome size (202 946 bp) compared to other known CMVs was not the result of an accidental deletion during passage in tissue culture. Surprisingly, the proteins encoded in MuHV-8 shared more overall similarity with their orthologues from mouse CMV (MuHV-1) compared to their orthologues in rat CMV (MuHV-2). Phylogenetic analyses of conserved viral genes showed that the two MuHV-8 isolates are from the same species and represent a unique clade that is distinct from other rodent CMVs.

Original language	English (US)
Pages (from-to)	1873-1882
Number of pages	10
Journal	Journal of General Virology
Volume	96
Issue number	7
DOIs	https://doi.org/10.1099/vir.0.000109
State	Published - Jul 1 2015

ASJC Scopus subject areas

Virology

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10.1099/vir.0.000109

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Geyer, H., Ettinger, J., Möller, L., Schmolz, E., Nitsche, A., Brune, W., Heaggans, S., Sandford, G. R., Hayward, G. S., & Voigt, S. (2015). Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV. Journal of General Virology, 96(7), 1873-1882. https://doi.org/10.1099/vir.0.000109

Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV. / Geyer, Henriette; Ettinger, Jakob; Möller, Lars et al.
In: Journal of General Virology, Vol. 96, No. 7, 01.07.2015, p. 1873-1882.

Research output: Contribution to journal › Article › peer-review

Geyer, H, Ettinger, J, Möller, L, Schmolz, E, Nitsche, A, Brune, W, Heaggans, S, Sandford, GR, Hayward, GS & Voigt, S 2015, 'Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV', Journal of General Virology, vol. 96, no. 7, pp. 1873-1882. https://doi.org/10.1099/vir.0.000109

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abstract = "The genome of the rat cytomegalovirus (RCMV) English isolate (MuHV-8) differs significantly from the RCMV Maastricht isolate (MuHV-2) and other cytomegaloviruses (CMVs) in its size, base composition and genomic content. Analysis of the RCMV-Berlin isolate, MuHV-8, revealed that the two MuHV-8 isolates are highly similar in genome size and content, indicating that the smaller genome size (202 946 bp) compared to other known CMVs was not the result of an accidental deletion during passage in tissue culture. Surprisingly, the proteins encoded in MuHV-8 shared more overall similarity with their orthologues from mouse CMV (MuHV-1) compared to their orthologues in rat CMV (MuHV-2). Phylogenetic analyses of conserved viral genes showed that the two MuHV-8 isolates are from the same species and represent a unique clade that is distinct from other rodent CMVs.",

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Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV

Abstract

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