ras Proteins Regulate Multiple Mitogenic Pathways in A10 Vascular Smooth Muscle Cells

K. Irani, S. Herzlinger, T. Finkel

Research output: Contribution to journalArticle

Abstract

We examined the role of ras proteins in mitogenesis and proliferation of A10 smooth muscle cells. An assay was developed in which a dominant negative ras gene could be transiently expressed in vascular smooth muscle cells. As opposed to cells transfected with a control plasmid, those transfected with a dominant negative ras expression plasmid exhibited a decrease in thymidine uptake in response to serum, platelet-derived growth factor, epidermal growth factor, fibroblast growth factor and thrombin stimulation. In addition, expression of dominant negative ras blocked smooth muscle cell proliferation as assessed by the number of surviving colonies after transfection and neomycin selection. Our data is the first to show that ras is essential for smooth muscle cell proliferation and is a mediator in multiple smooth muscle cell mitogenic signalling pathways.

Original languageEnglish (US)
Pages (from-to)1252-1258
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number3
DOIs
StatePublished - Aug 15 1994
Externally publishedYes

Fingerprint

ras Proteins
antineoplaston A10
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Cells
Cell proliferation
Plasmids
Cell Proliferation
Cell signaling
Neomycin
ras Genes
Fibroblast Growth Factors
Platelet-Derived Growth Factor
Epidermal Growth Factor
Thrombin
Thymidine
Transfection
Assays
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

ras Proteins Regulate Multiple Mitogenic Pathways in A10 Vascular Smooth Muscle Cells. / Irani, K.; Herzlinger, S.; Finkel, T.

In: Biochemical and Biophysical Research Communications, Vol. 202, No. 3, 15.08.1994, p. 1252-1258.

Research output: Contribution to journalArticle

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