RAS gene activation in acute myelogenous leukemia: Analysis by in vitro amplification and dna base sequence determination

Shrikant M. Mane, Stephen J. Meltzer, John C. Gutheil, Vikram Kapil, Edward J. Lee, Samuel W. Needleman

Research output: Contribution to journalArticlepeer-review

Abstract

RAS protooncogene activation has been repeatedly demonstrated in neoplastic cell DNA from patients with AML. Despite the convincing demonstration that activating RAS gene point mutations are critical in model systems, their precise prevalence and importance in human cancers such as AML remain speculative. The technology for identifying RAS mutations has changed considerably in recent years. We examined a prospective cohort of 43 acute myeloid leukemia (AML) patients admitted to the University of Maryland Cancer Center for first and second exon mutations of NRAS and KRAS using PCR and DNA sequence analysis. Six (14%) 1st exon NRAS mutations were identified. No clinical or biologic parameter has yet been observed to segregate with RAS activation, although a larger study may be needed to demonstrate this.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalGenes, Chromosomes and Cancer
Volume2
Issue number1
DOIs
StatePublished - May 1990
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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