Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.
- cell signaling
- chemically inducible dimerization
- reversible perturbation
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