Rapidly reversible manipulation of molecular activity with dual chemical dimerizers

Yu Chun Lin, Yuta Nihongaki, Tzu Yu Liu, Shiva Razavi, Moritoshi Sato, Takanari Inoue

Research output: Contribution to journalArticlepeer-review

Abstract

Tell it where to go: Rapamycin induced the relocation of an FRB-fused protein of interest (POI) to the plasma membrane (labeled with the fusion protein GAIs-FKBP-C2(LACT)) to activate a signaling event (see picture). Subsequent treatment with a gibberellic acid ester led to the relocation of the whole GAIs-FKBP-C2(LACT)/rapamycin/FRB-POI complex to the Tom20-GID1-labeled mitochondria with the termination of POI-dependent signaling.

Original languageEnglish (US)
Pages (from-to)6450-6454
Number of pages5
JournalAngewandte Chemie - International Edition
Volume52
Issue number25
DOIs
StatePublished - Jun 17 2013

Keywords

  • cell signaling
  • chemically inducible dimerization
  • enzymes
  • phosopholipids
  • reversible perturbation

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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