Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation

Hiroki Onuma, Toru Komatsu, Makoto Arita, Kenjiro Hanaoka, Tasuku Ueno, Takuya Terai, Tetsuo Nagano, Takanari Inoue

Research output: Contribution to journalArticle

Abstract

Cell surfaces represent a platform through which extracellular signals that determine diverse cellular processes, including migration, division, adhesion, and phagocytosis, are transduced. Techniques to rapidly reconfigure the surface properties of living cells should thus offer the ability to harness these cellular functions. Although the molecular mechanism of phagocytosis is well characterized, the minimal molecular players that are sufficient to activate this elaborate process remain elusive.We developed and implemented a technique to present a molecule of interest at the cell surface in an inducible manner on a time scale of minutes. We simultaneously induced the cell surface display of the C2 domain of milk fat globule epidermal growth factor factor 8 (MFG-E8) and activated the intracellular small guanosine triphosphatase Rac, which stimulates actin polymerization at the cell periphery. The C2 domain binds to phosphatidylserine, a lipid exposed on the surface of apoptotic cells. By integrating the stimulation of these two processes, we converted HeLa cells into a phagocytic cell line that bound to and engulfed apoptotic human Jurkat cells. Inducing either the cell surface display of the C2 domain or activating Rac alone was not sufficient to stimulate phagocytosis, which suggests that attachment to the target cell and actin reorganization together constitute the minimal molecular events that are needed to induce phagocytosis. This cell surface display technique might be useful as part of a targeted, cell-based therapy in which unwanted cells with characteristic surface molecules could be rapidly consumed by engineered cells.

Original languageEnglish (US)
Article numberrs4
JournalScience Signaling
Issue number334
DOIs
StatePublished - Jul 15 2014

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Cell Surface Display Techniques
Phagocytes
Chemical activation
Display devices
Phagocytosis
Cells
Actins
Molecules
Guanosine
Phosphatidylserines
Epidermal Growth Factor
Surface properties
Jurkat Cells
Surface Properties
Adhesion
Polymerization
Cell- and Tissue-Based Therapy
HeLa Cells
Lipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation. / Onuma, Hiroki; Komatsu, Toru; Arita, Makoto; Hanaoka, Kenjiro; Ueno, Tasuku; Terai, Takuya; Nagano, Tetsuo; Inoue, Takanari.

In: Science Signaling, No. 334, rs4, 15.07.2014.

Research output: Contribution to journalArticle

Onuma, Hiroki ; Komatsu, Toru ; Arita, Makoto ; Hanaoka, Kenjiro ; Ueno, Tasuku ; Terai, Takuya ; Nagano, Tetsuo ; Inoue, Takanari. / Rapidly rendering cells phagocytic through a cell surface display technique and concurrent Rac activation. In: Science Signaling. 2014 ; No. 334.
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