Rapidly relocating molecules between organelles to manipulate small gtpase activity

Siew Cheng Phua, Christopher Pohlmeyer, Takanari Inoue

Research output: Contribution to journalArticlepeer-review

Abstract

Chemically inducible rapid manipulation of small GTPase activity has proven a powerful approach to dissect complex spatiotemporal signaling of these molecular switches. However, overexpression of these synthetic molecular probes freely in the cytosol often results in elevated background activity before chemical induction, which perturbs the cellular basal state and thereby limits their wide application. As a fundamental solution, we have rationally designed and newly developed a strategy to remove unwanted background activity without compromising the extent of induced activation. By exploiting interaction between a membrane lipid and its binding protein, target proteins were translocated from one organelle to another on a time scale of seconds. This improved strategy now allows for rapid manipulation of small GTPases under a physiological state, thus enabling fine dissection of sophisticated signaling processes shaped by these molecules.

Original languageEnglish (US)
Pages (from-to)1950-1955
Number of pages6
JournalACS chemical biology
Volume7
Issue number12
DOIs
StatePublished - Dec 21 2012

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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