TY - JOUR
T1 - Rapidly released allergens from short ragweed pollen. II. Identification and partial purification
AU - Hussain, Rabia
AU - Norman, Philip S.
AU - Marsh, David G.
N1 - Funding Information:
From -he Laboratory of the Howard Hughes Medical Institute in the DiGsiono f Clinical ImmunologyD, epartmenotf Medicine,T he Jo&x Hopkins University School of Medicine at the Good Samaritaa Hospital. Supprted by National Institutes of Health grants AI-09565, 1?!70, and AI-04866 and Food and Drug Administration trait 73-156. Retivcd for publication May 27, 1980. Accepted for publication Oct. 30, 1980. Rep& requests to: David G. Marsh, Ph.D., The Good Samaritan Hwpital, 5601 Loch Raven Blvd., Baltimore, MD 21239. *PuWicarion No. 418, O’Neill Research Laboratories, Sam&an Hospital. Baltimore, Md. **&sent address: Lakxatory of Parasitic Diseases, Bldg. 5, Rm. BL.30, National Institute of Alkrgy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20014.
PY - 1981/3
Y1 - 1981/3
N2 - We have identified several basic allergens in 16-min extracts of short ragweed (Ambrosia elatior) pollen and obtained a partially purified fraction (G50 II) using a combination of ion-exchange (CM-Sephadex C25) and gel-filtration (Biogel P30 and Sephadex G50) procedures. G50 II was allergenically and antigenically distinct from known allergens antigen E, Ra3, and Ra5. It gave a highly symmetrical peak on Sephadex G50 (fine), corresponding to a molecular weight of 11,500 daltons, and a single band on SDS polyacrylamide gel electrophoresis under both reducing and nonreducing conditions. However, two cathodic antigens were detectable by both agarose electrophoresis and crossed immunoelectrophoresis. Allergenic activity of G50 II, assessed by puncture testing in ragweed-sensitive subjects, showed a characteristic pattern of response independent of patient response to AgE, Ra3, and Ra5. The major antigenic and allergenic component of G50 II is designated Ra6.
AB - We have identified several basic allergens in 16-min extracts of short ragweed (Ambrosia elatior) pollen and obtained a partially purified fraction (G50 II) using a combination of ion-exchange (CM-Sephadex C25) and gel-filtration (Biogel P30 and Sephadex G50) procedures. G50 II was allergenically and antigenically distinct from known allergens antigen E, Ra3, and Ra5. It gave a highly symmetrical peak on Sephadex G50 (fine), corresponding to a molecular weight of 11,500 daltons, and a single band on SDS polyacrylamide gel electrophoresis under both reducing and nonreducing conditions. However, two cathodic antigens were detectable by both agarose electrophoresis and crossed immunoelectrophoresis. Allergenic activity of G50 II, assessed by puncture testing in ragweed-sensitive subjects, showed a characteristic pattern of response independent of patient response to AgE, Ra3, and Ra5. The major antigenic and allergenic component of G50 II is designated Ra6.
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U2 - 10.1016/0091-6749(81)90064-6
DO - 10.1016/0091-6749(81)90064-6
M3 - Article
C2 - 7462538
AN - SCOPUS:0019463307
SN - 0091-6749
VL - 67
SP - 217
EP - 222
JO - The Journal of allergy and clinical immunology
JF - The Journal of allergy and clinical immunology
IS - 3
ER -