Rapid transdermal bloodless and reagent-free malaria detection

Ekaterina Y. Lukianova-Hleb, Kelly M. Campbell, Pamela E. Constantinou, Janet Braam, John S. Olson, Russell E. Ware, David J Sullivan, Dmitri Lapotko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Successful diagnosis, screening, and elimination of malaria critically depend on rapid and sensitive detection of this dangerous infection, preferably transdermally and without sophisticated reagents or blood drawing. Such diagnostic methods are not currently available. Here we show that the high optical absorbance and nanosize of endogenous heme nanoparticles called hemozoin, a unique component of all blood-stage malaria parasites, generate a transient vapor nanobubble around hemozoin in response to a short and safe near-infrared picosecond laser pulse. The acoustic signals of these malaria-specific nanobubbles provided the first transdermal non-invasive and rapid detection of a malaria infection as low as 0.00034% in animals without using any reagents or drawing blood. These on-demand transient events have no analogs among current malaria markers and probes, can detect and screen malaria in seconds and can be realized as a compact, easy to use, inexpensive and safe field technology.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8935
ISBN (Print)9780819498489
DOIs
StatePublished - 2014
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII - San Francisco, CA, United States
Duration: Feb 2 2014Feb 4 2014

Other

OtherAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII
CountryUnited States
CitySan Francisco, CA
Period2/2/142/4/14

Fingerprint

Malaria
blood
reagents
Blood
infectious diseases
parasites
Infrared lasers
Heme
markers
animals
elimination
Laser pulses
Screening
Animals
screening
Acoustics
Vapors
vapors
analogs
Nanoparticles

Keywords

  • Hemozoin
  • Laser
  • Malaria
  • Vapor nanobubble

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Lukianova-Hleb, E. Y., Campbell, K. M., Constantinou, P. E., Braam, J., Olson, J. S., Ware, R. E., ... Lapotko, D. (2014). Rapid transdermal bloodless and reagent-free malaria detection. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8935). [893509] SPIE. https://doi.org/10.1117/12.2040695

Rapid transdermal bloodless and reagent-free malaria detection. / Lukianova-Hleb, Ekaterina Y.; Campbell, Kelly M.; Constantinou, Pamela E.; Braam, Janet; Olson, John S.; Ware, Russell E.; Sullivan, David J; Lapotko, Dmitri.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8935 SPIE, 2014. 893509.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lukianova-Hleb, EY, Campbell, KM, Constantinou, PE, Braam, J, Olson, JS, Ware, RE, Sullivan, DJ & Lapotko, D 2014, Rapid transdermal bloodless and reagent-free malaria detection. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8935, 893509, SPIE, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XII, San Francisco, CA, United States, 2/2/14. https://doi.org/10.1117/12.2040695
Lukianova-Hleb EY, Campbell KM, Constantinou PE, Braam J, Olson JS, Ware RE et al. Rapid transdermal bloodless and reagent-free malaria detection. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8935. SPIE. 2014. 893509 https://doi.org/10.1117/12.2040695
Lukianova-Hleb, Ekaterina Y. ; Campbell, Kelly M. ; Constantinou, Pamela E. ; Braam, Janet ; Olson, John S. ; Ware, Russell E. ; Sullivan, David J ; Lapotko, Dmitri. / Rapid transdermal bloodless and reagent-free malaria detection. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8935 SPIE, 2014.
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