Rapid, targeted and culture-free viral infectivity assay in drop-based microfluidics

Ye Tao, Assaf Rotem, Huidan Zhang, Connie B. Chang, Anindita Basu, Abimbola O. Kolawole, Stephan A. Koehler, Yukun Ren, Jeffrey S. Lin, James M. Pipas, Andrew B. Feldman, Christiane E. Wobus, David A. Weitz

Research output: Contribution to journalArticle

Abstract

A key viral property is infectivity, and its accurate measurement is crucial for the understanding of viral evolution, disease and treatment. Currently viral infectivity is measured using plaque assays, which involve prolonged culturing of host cells, and whose measurement is unable to differentiate between specific strains and is prone to low number fluctuation. We developed a rapid, targeted and culture-free infectivity assay using high-throughput drop-based microfluidics. Single infectious viruses are incubated in a large number of picoliter drops with host cells for one viral replication cycle followed by in-drop gene-specific amplification to detect infection events. Using murine noroviruses (MNV) as a model system, we measure their infectivity and determine the efficacy of a neutralizing antibody for different variants of MNV. Our results are comparable to traditional plaque-based assays and plaque reduction neutralization tests. However, the fast, low-cost, highly accurate genomic-based assay promises to be a superior method for drug screening and isolation of resistant viral strains. Moreover our technique can be adapted to measuring the infectivity of other pathogens, such as bacteria and fungi.

Original languageEnglish (US)
Pages (from-to)3934-3940
Number of pages7
JournalLab on a Chip
Volume15
Issue number19
DOIs
StatePublished - Aug 14 2015

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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  • Cite this

    Tao, Y., Rotem, A., Zhang, H., Chang, C. B., Basu, A., Kolawole, A. O., Koehler, S. A., Ren, Y., Lin, J. S., Pipas, J. M., Feldman, A. B., Wobus, C. E., & Weitz, D. A. (2015). Rapid, targeted and culture-free viral infectivity assay in drop-based microfluidics. Lab on a Chip, 15(19), 3934-3940. https://doi.org/10.1039/c5lc00556f