Rapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route

Mary Lynn Duckworth, Michael J. Gait, Philip Goelet, Guo Fang Hong, Mohinder Singh, Richard C. Titmas

Research output: Contribution to journalArticlepeer-review

Abstract

Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.

Original languageEnglish (US)
Pages (from-to)1691-1706
Number of pages16
JournalNucleic acids research
Volume9
Issue number7
DOIs
StatePublished - Apr 10 1981
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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