Abstract
A rapid molecular screening programme has been established for the long arm of the human Y chromosome in Yq11 in order to quickly detect small interstitial deletions in this chromosome region. They have been observed in idiopathic sterile males with azoospermia and a severe oligozoospermia and are therefore indicative for deletion of AZF gene sequences. AZF (i.e. azoospermia factor) is a genetic factor located in Yq11 which controls human spermatogenesis. The screening programme is based mainly on a multiplex PCR approach using a series of Y-specific primers amplifying single DNA loci in Yq11. The order of all Y-DNA loci can be unequivocally arranged along the whole long Y arm. Therefore, any detected deletion can be quickly mapped in relation to the proposed position of AZF. Benefits and pitfalls of this new diagnostic Y screening method will be discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 97-106 |
| Number of pages | 10 |
| Journal | Andrologia |
| Volume | 26 |
| Issue number | 2 |
| State | Published - 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Endocrinology
- Nephrology
Cite this
Rapid screening of the Y chromosome in idiopathic sterile men, diagnostic for deletions in AZF, a genetic Y factor expressed during spermatogenesis. / Henegariu, O.; Hirschmann, P.; Kilian, K.; Kirsch, S.; Lengauer, C.; Maiwald, R.; Mielke, K.; Vogt, P.
In: Andrologia, Vol. 26, No. 2, 1994, p. 97-106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid screening of the Y chromosome in idiopathic sterile men, diagnostic for deletions in AZF, a genetic Y factor expressed during spermatogenesis
AU - Henegariu, O.
AU - Hirschmann, P.
AU - Kilian, K.
AU - Kirsch, S.
AU - Lengauer, C.
AU - Maiwald, R.
AU - Mielke, K.
AU - Vogt, P.
PY - 1994
Y1 - 1994
N2 - A rapid molecular screening programme has been established for the long arm of the human Y chromosome in Yq11 in order to quickly detect small interstitial deletions in this chromosome region. They have been observed in idiopathic sterile males with azoospermia and a severe oligozoospermia and are therefore indicative for deletion of AZF gene sequences. AZF (i.e. azoospermia factor) is a genetic factor located in Yq11 which controls human spermatogenesis. The screening programme is based mainly on a multiplex PCR approach using a series of Y-specific primers amplifying single DNA loci in Yq11. The order of all Y-DNA loci can be unequivocally arranged along the whole long Y arm. Therefore, any detected deletion can be quickly mapped in relation to the proposed position of AZF. Benefits and pitfalls of this new diagnostic Y screening method will be discussed.
AB - A rapid molecular screening programme has been established for the long arm of the human Y chromosome in Yq11 in order to quickly detect small interstitial deletions in this chromosome region. They have been observed in idiopathic sterile males with azoospermia and a severe oligozoospermia and are therefore indicative for deletion of AZF gene sequences. AZF (i.e. azoospermia factor) is a genetic factor located in Yq11 which controls human spermatogenesis. The screening programme is based mainly on a multiplex PCR approach using a series of Y-specific primers amplifying single DNA loci in Yq11. The order of all Y-DNA loci can be unequivocally arranged along the whole long Y arm. Therefore, any detected deletion can be quickly mapped in relation to the proposed position of AZF. Benefits and pitfalls of this new diagnostic Y screening method will be discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028360261&partnerID=8YFLogxK
M3 - Article
VL - 26
SP - 97
EP - 106
JO - Andrologia
JF - Andrologia
SN - 0303-4569
IS - 2
ER -