Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1

Judith E. Beach, Robert C. Smallridge, Carol A. Kinzer, Edward W. Bernton, John W. Holaday, Henry G. Fein

Research output: Contribution to journalArticle

Abstract

Previous studies demonstrated a direct action of interleukin-1 (IL-1) on release of hormones from rat anterior pituitary cells in monolayer culture. To rule out any possibility of a paracrine effect from the elevated hormones in the static monolayer system, and to examine further the dynamics of hormone release elicited by IL-1, studies were conducted with rat anterior pituitary tissue in a computer-controlled automated perifusion system. In experiments performed on the same day as sacrifice, IL-1 stimulated the release of adrenocorticotrophic hormone (ACTH), luteinizing hormone (LH), thyroid stimulating hormone (TSH), growth hormone (GH) and prolactin (PRL) in a dose-related manner. Peak levels were achieved within 6 minutes of exposure to IL-1. However, PRL was not increased over the baseline fluctuations when pituitaries were perifused with IL-1 after 72 hours of incubation. Hormone release did not appear to undergo desensitization after multiple short pulses of IL-1. Heat-denatured IL-1 had no effect on hormone release. The rapid response suggests that IL-1 acts acutely to release preformed hormone stores.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalLife Sciences
Volume44
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Pituitary Hormones
Interleukin-1
Rats
Hormones
Prolactin
Monolayers
Anterior Pituitary Hormones
Thyrotropin
Luteinizing Hormone
Adrenocorticotropic Hormone
Growth Hormone
Hot Temperature
Tissue

ASJC Scopus subject areas

  • Pharmacology

Cite this

Beach, J. E., Smallridge, R. C., Kinzer, C. A., Bernton, E. W., Holaday, J. W., & Fein, H. G. (1989). Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1. Life Sciences, 44(1), 1-7. https://doi.org/10.1016/0024-3205(89)90211-7

Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1. / Beach, Judith E.; Smallridge, Robert C.; Kinzer, Carol A.; Bernton, Edward W.; Holaday, John W.; Fein, Henry G.

In: Life Sciences, Vol. 44, No. 1, 1989, p. 1-7.

Research output: Contribution to journalArticle

Beach, JE, Smallridge, RC, Kinzer, CA, Bernton, EW, Holaday, JW & Fein, HG 1989, 'Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1', Life Sciences, vol. 44, no. 1, pp. 1-7. https://doi.org/10.1016/0024-3205(89)90211-7
Beach, Judith E. ; Smallridge, Robert C. ; Kinzer, Carol A. ; Bernton, Edward W. ; Holaday, John W. ; Fein, Henry G. / Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1. In: Life Sciences. 1989 ; Vol. 44, No. 1. pp. 1-7.
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