Rapid Quantitation of desmosine content in tissue hydrolysates by high-performance liquid chromatography

Barbara Faris, Rocco Ferrera, Michael Glembourtt, Peter J. Mogayzel, George Crombie, Carl Franzblau

Research output: Contribution to journalArticlepeer-review

Abstract

The lysine-derived crosslinks in elastin, desmosine, and isodesmosine, are quantitated in tissue hydrolysates by monitoring high-performance liquid chromatography eluents at 275 nm. The results from this method compare favorably with results from the amino acid analyzer. However, this more sensitive method (1) eliminates ninhydrin-positive artifacts which elute with the desmosines from some tissue hydrolysates on the amino acid analyzer, and (2) makes possible elastin quantitation in a tissue with the minimum amount of manipulations.

Original languageEnglish (US)
Pages (from-to)71-74
Number of pages4
JournalAnalytical biochemistry
Volume114
Issue number1
DOIs
StatePublished - Jun 1981
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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