TY - JOUR
T1 - Rapid Quantitation of desmosine content in tissue hydrolysates by high-performance liquid chromatography
AU - Faris, Barbara
AU - Ferrera, Rocco
AU - Glembourtt, Michael
AU - Mogayzel, Peter J.
AU - Crombie, George
AU - Franzblau, Carl
PY - 1981/6
Y1 - 1981/6
N2 - The lysine-derived crosslinks in elastin, desmosine, and isodesmosine, are quantitated in tissue hydrolysates by monitoring high-performance liquid chromatography eluents at 275 nm. The results from this method compare favorably with results from the amino acid analyzer. However, this more sensitive method (1) eliminates ninhydrin-positive artifacts which elute with the desmosines from some tissue hydrolysates on the amino acid analyzer, and (2) makes possible elastin quantitation in a tissue with the minimum amount of manipulations.
AB - The lysine-derived crosslinks in elastin, desmosine, and isodesmosine, are quantitated in tissue hydrolysates by monitoring high-performance liquid chromatography eluents at 275 nm. The results from this method compare favorably with results from the amino acid analyzer. However, this more sensitive method (1) eliminates ninhydrin-positive artifacts which elute with the desmosines from some tissue hydrolysates on the amino acid analyzer, and (2) makes possible elastin quantitation in a tissue with the minimum amount of manipulations.
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U2 - 10.1016/0003-2697(81)90453-X
DO - 10.1016/0003-2697(81)90453-X
M3 - Article
C2 - 7283156
AN - SCOPUS:0019798357
SN - 0003-2697
VL - 114
SP - 71
EP - 74
JO - Analytical biochemistry
JF - Analytical biochemistry
IS - 1
ER -