Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column

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Abstract

A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalAnalytical Biochemistry
Volume160
Issue number1
DOIs
StatePublished - 1987

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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