Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column

Bert Vogelstein

doi:10.1016/0003-2697(87)90620-8

Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column

Bert Vogelstein

School of Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.

Original language	English (US)
Pages (from-to)	115-118
Number of pages	4
Journal	Analytical biochemistry
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(87)90620-8
State	Published - Jan 1987

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(87)90620-8

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title = "Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column",

abstract = "A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.",

author = "Bert Vogelstein",

note = "Funding Information: The author thanks B. Zehnbauer, A. Wang, K. Rinzler, and E. R. Fearon for helpful suggestions, and S. Smith for preparing the manuscript. This work was sup ported by the Clayton Fund and by Grants CA35494 and CA43460, awarded by the NIH, DHHS.",

year = "1987",

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PY - 1987/1

Y1 - 1987/1

N2 - A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.

AB - A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.

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JO - Analytical Biochemistry

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ER -

Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column

Abstract

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