Rapid multiple-determinant enzyme immunoassay for the detection of human rotavirus

Robert H Yolken, F. Leister

Research output: Contribution to journalArticle

Abstract

Enzyme immunoassays (EIAs) can be used to detect rotavirus antigens accurately in human stool specimens. Conventional EIA systems require prolonged incubations and multiple washing steps, thus making them impractical for truly rapid diagnosis. However, more rapid solid-phase EIA systems can be devised which make use of simultaneous binding of enzyme-labeled and solid-phase antibodies at immunologically distinct antigenic sites. A multiple-determinant EIA system was devised that was capable of detecting small quantities of rotavirus antigen in

Original languageEnglish (US)
Pages (from-to)43-46
Number of pages4
JournalJournal of Infectious Diseases
Volume146
Issue number1
StatePublished - 1982

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Rotavirus
Immunoenzyme Techniques
Antigens
Antibodies
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Rapid multiple-determinant enzyme immunoassay for the detection of human rotavirus. / Yolken, Robert H; Leister, F.

In: Journal of Infectious Diseases, Vol. 146, No. 1, 1982, p. 43-46.

Research output: Contribution to journalArticle

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