Rapid microbiological assay for chloramphenicol and tetracyclines

T. J. Louie, F. P. Tally, J. G. Bartlett, S. L. Gorbach

Research output: Contribution to journalArticlepeer-review

Abstract

An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well defined zones formed after a 1.5 to 2 h incubation in an anaerobic chamber or after 3 h using a GasPak system. Serum levels of chloramphenicol as low as 2.0 μg/ml could be reproducibly determined with this method. Concurrent presence of gentamicin did not influence the assay. Levels of penicillin G were also reproducibly determined with this method, although use of stable zones of growth inhibition which would form after overnight incubation were a more convenient end point for this bactericidal drug. The hemolytic assay with C. perfringens is a reproducible, accurate, and rapid method for determination of levels for chloramphenicol and tetracyclines.

Original languageEnglish (US)
Pages (from-to)874-878
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume9
Issue number6
DOIs
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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