Rapid liquid-liquid extraction of cocaine from urine for gas chromatographic-mass spectrometric analysis

Diana Garside, Bruce A. Goldberger, Kenzie L. Preston, Edward J. Cone

Research output: Contribution to journalArticle

Abstract

A novel, simple and economic liquid-liquid extraction method for isolating cocaine from urine was developed utilizing gas chromatography-mass spectrometry (GC-MS) for analysis and quantification. The use of a single nonpolar organic solvent allowed only nonpolar analytes to be extracted from the biological fluid, and consequently, no derivatization step was necessary before CC-MS analysis. Large numbers of specimens (>60) can be extracted in approximately 3 h with this procedure. The method is highly precise (C.V. 98%), sensitive (limit of detection of 5 ng/ml) and has a mean recovery of 48.8%.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume692
Issue number1
DOIs
StatePublished - Apr 25 1997
Externally publishedYes

Fingerprint

Cocaine
Gases
Liquids
Gas chromatography
Organic solvents
Mass spectrometry
Recovery
Economics
Fluids

Keywords

  • Cocaine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Rapid liquid-liquid extraction of cocaine from urine for gas chromatographic-mass spectrometric analysis. / Garside, Diana; Goldberger, Bruce A.; Preston, Kenzie L.; Cone, Edward J.

In: Journal of Chromatography B: Biomedical Applications, Vol. 692, No. 1, 25.04.1997, p. 61-65.

Research output: Contribution to journalArticle

Garside, Diana ; Goldberger, Bruce A. ; Preston, Kenzie L. ; Cone, Edward J. / Rapid liquid-liquid extraction of cocaine from urine for gas chromatographic-mass spectrometric analysis. In: Journal of Chromatography B: Biomedical Applications. 1997 ; Vol. 692, No. 1. pp. 61-65.
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