Rapid isolation and characterization of amplified DNA by chromosome microdissection: Identification of IGF1R amplification in malignant melanoma

J. Zhang, J. M. Trent, P. S. Meltzer

Research output: Contribution to journalArticle

Abstract

We describe a novel strategy characterizing gene amplification in human neoplasia based on targeting double minutes (dmin) and homogeneously staining regions (hsr) for chromosome microdissection. This strategy allows the rapid generation of an amplification unit microclone library and permits the rapid identification of the chromosomal origin of the amplified sequences following fluorescence in situ hybridization (FISH). This strategy has been applied to an hsr-bearing malignant melanoma cell line, HA-A, which was then demonstrated to encode multiple overexpressed copies of the IGF1R gene. This strategy combines all steps for detection, cloning, mapping and isolation of amplified gene(s) into a single process that is readily applicable to any specimen carrying cytologic evidence of gene amplification.

Original languageEnglish (US)
Pages (from-to)2827-2831
Number of pages5
JournalOncogene
Volume8
Issue number10
StatePublished - 1993

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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