Rapid increases in peptide processing enzyme expression in hippocampal neurons

Ratan V. Bhat, Francisco A. Tausk, Jay M Baraban, Richard E. Mains, Betty A. Eipper

Research output: Contribution to journalArticle

Abstract

Recent studies have demonstrated that seizure activity causes a dramatic increase in neuropeptide expression in specific regions of the rat hippocampus. In this study we investigated the effect of electroconvulsive treatment (ECT) on the expression of three posttranslational processing enzymes involved in the production of many bioactive peptides from their inactive precursors. Peptidylglycine α-amidating monooxygenase (PAM) converts peptidylglycine substrates into α-amidated products and prohormone convertases 1 and 2 perform the tissue-specific endoproteolytic cleavage of many prohormones. After a single ECT, in situ hybridization demonstrated a rapid increase in the level of PAM mRNA in the dentate granule cells of the hippocampus, reaching peak levels between 1 and 4 h and then returning to near baseline levels within 24 h. Northern blot analysis confirmed the changes in PAM mRNA expression seen by using in situ hybridization. Similar rapid changes in PAM mRNA expression were seen after repeated ECT, suggesting that chronic ECT did not affect the regulation of PAM expression in the hippocampus. Immunohistochemical staining demonstrated an increase in PAM protein in the molecular layer of the dentate gyrus at 4 and 8 h after a single ECT. Based on in situ hybridization, levels of mRNA for the prohormone convertases 1 and 2 were also increased in dentate granule cells after a single ECT. Prohormone convertase 2 mRNA levels exhibited a slower response to ECT, not reaching maximal levels until 8 h after ECT. The response of the dentate granule cells of the hippocampus to ECT provides a model system for studying the rapid, coordinate regulation of peptide-processing enzymes.

Original languageEnglish (US)
Pages (from-to)1315-1322
Number of pages8
JournalJournal of Neurochemistry
Volume61
Issue number4
StatePublished - Oct 1993

Fingerprint

Mixed Function Oxygenases
Proprotein Convertase 2
Neurons
Proprotein Convertase 1
Messenger RNA
Peptides
Hippocampus
Enzymes
Processing
In Situ Hybridization
Neuropeptides
Dentate Gyrus
Rats
Northern Blotting
peptidylglycine monooxygenase
Tissue
Seizures
Staining and Labeling
Substrates
Proteins

Keywords

  • Amidation
  • Electroconvulsive treatment
  • Hippocampus
  • Neuropeptide
  • Posttranslational processing enzyme
  • Prohormone convertase
  • Seizure

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Bhat, R. V., Tausk, F. A., Baraban, J. M., Mains, R. E., & Eipper, B. A. (1993). Rapid increases in peptide processing enzyme expression in hippocampal neurons. Journal of Neurochemistry, 61(4), 1315-1322.

Rapid increases in peptide processing enzyme expression in hippocampal neurons. / Bhat, Ratan V.; Tausk, Francisco A.; Baraban, Jay M; Mains, Richard E.; Eipper, Betty A.

In: Journal of Neurochemistry, Vol. 61, No. 4, 10.1993, p. 1315-1322.

Research output: Contribution to journalArticle

Bhat, RV, Tausk, FA, Baraban, JM, Mains, RE & Eipper, BA 1993, 'Rapid increases in peptide processing enzyme expression in hippocampal neurons', Journal of Neurochemistry, vol. 61, no. 4, pp. 1315-1322.
Bhat, Ratan V. ; Tausk, Francisco A. ; Baraban, Jay M ; Mains, Richard E. ; Eipper, Betty A. / Rapid increases in peptide processing enzyme expression in hippocampal neurons. In: Journal of Neurochemistry. 1993 ; Vol. 61, No. 4. pp. 1315-1322.
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