TY - JOUR
T1 - Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry
AU - Chen, Kuan Fu
AU - Rothman, Richard E.
AU - Ramachandran, Padmini
AU - Blyn, Lawrence
AU - Sampath, Rangarajan
AU - Ecker, David J.
AU - Valsamakis, Alexandra
AU - Gaydos, Charlotte A.
N1 - Funding Information:
Grant numbers and sources of support: Drs. Gaydos and Rothman are supported in part by NIAID 2U54AI057168.
PY - 2011/4
Y1 - 2011/4
N2 - Diagnosis of the etiologic agent of respiratory viral infection relies traditionally on culture or antigen detection. This pilot evaluation compared performance characteristics of the RT-PCR and electrospray ionization mass spectrometry (RT-PCR/ESI-MS) platform to conventional virologic methods for identifying multiple clinically relevant respiratory viruses in nasopharyngeal aspirates. The RT-PCR/ESI-MS respiratory virus surveillance kit was designed to detect respiratory syncytial virus, influenza A and B, parainfluenza types 1-4, adenoviridae types A-F, coronaviridae, human bocavirus, and human metapneumovirus. Patients (. N=192) attending an emergency department during the 2007-2008 respiratory season consented, and " excess" frozen archived nasopharyngeal aspirates were analysed; 46 were positive by conventional virology and 69 by RT-PCR/ESI-MS, among which there were six samples with multiple viral pathogens detected. The sensitivity and specificity of the assay were 89.1% and 80.3%, respectively. Additional viruses that were not identified by conventional virology assays were detected (4 human bocaviruses and 7 coronaviruses). Samples in which the RT-PCR/ESI-MS results disagreed with conventional virology were sent for analysis by a third method using a commercial RT-PCR-based assay, which can identify viruses not detectable by conventional virologic procedures. Time to first result of RT-PCR/ESI-MS was 8. h. RT-PCR/ESI-MS demonstrated capacity to detect respiratory viruses identifiable and unidentifiable by conventional methods rapidly.
AB - Diagnosis of the etiologic agent of respiratory viral infection relies traditionally on culture or antigen detection. This pilot evaluation compared performance characteristics of the RT-PCR and electrospray ionization mass spectrometry (RT-PCR/ESI-MS) platform to conventional virologic methods for identifying multiple clinically relevant respiratory viruses in nasopharyngeal aspirates. The RT-PCR/ESI-MS respiratory virus surveillance kit was designed to detect respiratory syncytial virus, influenza A and B, parainfluenza types 1-4, adenoviridae types A-F, coronaviridae, human bocavirus, and human metapneumovirus. Patients (. N=192) attending an emergency department during the 2007-2008 respiratory season consented, and " excess" frozen archived nasopharyngeal aspirates were analysed; 46 were positive by conventional virology and 69 by RT-PCR/ESI-MS, among which there were six samples with multiple viral pathogens detected. The sensitivity and specificity of the assay were 89.1% and 80.3%, respectively. Additional viruses that were not identified by conventional virology assays were detected (4 human bocaviruses and 7 coronaviruses). Samples in which the RT-PCR/ESI-MS results disagreed with conventional virology were sent for analysis by a third method using a commercial RT-PCR-based assay, which can identify viruses not detectable by conventional virologic procedures. Time to first result of RT-PCR/ESI-MS was 8. h. RT-PCR/ESI-MS demonstrated capacity to detect respiratory viruses identifiable and unidentifiable by conventional methods rapidly.
KW - Diagnosis
KW - Electrospray ionization mass spectrometry
KW - Respiratory tract infections
KW - Surveillance
KW - Virus
UR - http://www.scopus.com/inward/record.url?scp=79952184903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952184903&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2011.01.007
DO - 10.1016/j.jviromet.2011.01.007
M3 - Article
C2 - 21256867
AN - SCOPUS:79952184903
SN - 0166-0934
VL - 173
SP - 60
EP - 66
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -