Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry

Kuan Fu Chen, Richard E. Rothman, Padmini Ramachandran, Lawrence Blyn, Rangarajan Sampath, David J. Ecker, Alexandra Valsamakis, Charlotte A. Gaydos

Research output: Contribution to journalArticle

Abstract

Diagnosis of the etiologic agent of respiratory viral infection relies traditionally on culture or antigen detection. This pilot evaluation compared performance characteristics of the RT-PCR and electrospray ionization mass spectrometry (RT-PCR/ESI-MS) platform to conventional virologic methods for identifying multiple clinically relevant respiratory viruses in nasopharyngeal aspirates. The RT-PCR/ESI-MS respiratory virus surveillance kit was designed to detect respiratory syncytial virus, influenza A and B, parainfluenza types 1-4, adenoviridae types A-F, coronaviridae, human bocavirus, and human metapneumovirus. Patients (. N=192) attending an emergency department during the 2007-2008 respiratory season consented, and " excess" frozen archived nasopharyngeal aspirates were analysed; 46 were positive by conventional virology and 69 by RT-PCR/ESI-MS, among which there were six samples with multiple viral pathogens detected. The sensitivity and specificity of the assay were 89.1% and 80.3%, respectively. Additional viruses that were not identified by conventional virology assays were detected (4 human bocaviruses and 7 coronaviruses). Samples in which the RT-PCR/ESI-MS results disagreed with conventional virology were sent for analysis by a third method using a commercial RT-PCR-based assay, which can identify viruses not detectable by conventional virologic procedures. Time to first result of RT-PCR/ESI-MS was 8. h. RT-PCR/ESI-MS demonstrated capacity to detect respiratory viruses identifiable and unidentifiable by conventional methods rapidly.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalJournal of Virological Methods
Volume173
Issue number1
DOIs
StatePublished - Apr 1 2011

Keywords

  • Diagnosis
  • Electrospray ionization mass spectrometry
  • Respiratory tract infections
  • Surveillance
  • Virus

ASJC Scopus subject areas

  • Virology

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