Rapid identification of Vibrio cholerae serotype O1 from primary isolation plates by a coagglutination test

M. Rahman, D. A. Sack, A. Wadood, M. Yasmin, A. Latif

Research output: Contribution to journalArticle

Abstract

A coagglutination test was developed for identifying suspected colonies of Vibrio cholerae serotype O1 directly from primary isolation plates. Visible agglutination occurs when V. cholerae O1 antibody attached to cell-wall protein A of Staphylococcus aureus reacts with its homologous antigen. From 314 faecal samples from clinically suspected cases of cholera, 210 colonies from thiosulphate citrate bile salts sucrose (TCBS) agar and 222 colonies from taurocholate tellurite gelatin (TTG) agar were tested as suspect V. cholerae. In each case 204 isolates were identified as V. cholerae O1 by conventional methods and also gave positive results for V. cholerae O1 in the coagglutination test; with one partial exception, no other colonies tested gave positive results. The coagglutination test is simple and inexpensive and provides a result 24 h earlier than conventional methods.

Original languageEnglish (US)
Pages (from-to)39-41
Number of pages3
JournalJournal of Medical Microbiology
Volume28
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Vibrio cholerae O1
Agar
Thiosulfates
Taurocholic Acid
Vibrio cholerae
Cholera
Agglutination
Staphylococcal Protein A
Gelatin
Bile Acids and Salts
Citric Acid
Cell Wall
Sucrose
Staphylococcus aureus
Antigens
Serogroup
Antibodies

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Rapid identification of Vibrio cholerae serotype O1 from primary isolation plates by a coagglutination test. / Rahman, M.; Sack, D. A.; Wadood, A.; Yasmin, M.; Latif, A.

In: Journal of Medical Microbiology, Vol. 28, No. 1, 1989, p. 39-41.

Research output: Contribution to journalArticle

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