Rapid identification of Clostridium botulinum colonies by in vitro toxicity and antimicrobial susceptibility testing

M. Dezfulian; J. G. Bartlett

doi:10.1007/BF00357557

Rapid identification of Clostridium botulinum colonies by in vitro toxicity and antimicrobial susceptibility testing

Research output: Contribution to journal › Article › peer-review

Abstract

Identification of Clostridium botulinum is usually based on toxin detection of broth culture by mouse bioassay and requires 7 to 10 days to complete. Here, we describe an alternative in vitro procedure for direct identification of C. botulinum (types A and B) colonies which can be completed in 48 h. The method is based on toxigenicity of colonies demonstrable by enzyme immunoassay and resistance of C. botulinum to antimicrobial agents, sulpha-methoxazole, trimethoprim and cycloserine.

Original language	English (US)
Pages (from-to)	27-29
Number of pages	3
Journal	World Journal of Microbiology & Biotechnology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF00357557
State	Published - Jan 1 1994
Externally published	Yes

Keywords

Clostridium botulinum
identification
immunoassay
resistance
toxigenicity

ASJC Scopus subject areas

Biotechnology
Physiology
Applied Microbiology and Biotechnology

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10.1007/BF00357557

Cite this

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abstract = "Identification of Clostridium botulinum is usually based on toxin detection of broth culture by mouse bioassay and requires 7 to 10 days to complete. Here, we describe an alternative in vitro procedure for direct identification of C. botulinum (types A and B) colonies which can be completed in 48 h. The method is based on toxigenicity of colonies demonstrable by enzyme immunoassay and resistance of C. botulinum to antimicrobial agents, sulpha-methoxazole, trimethoprim and cycloserine.",

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N2 - Identification of Clostridium botulinum is usually based on toxin detection of broth culture by mouse bioassay and requires 7 to 10 days to complete. Here, we describe an alternative in vitro procedure for direct identification of C. botulinum (types A and B) colonies which can be completed in 48 h. The method is based on toxigenicity of colonies demonstrable by enzyme immunoassay and resistance of C. botulinum to antimicrobial agents, sulpha-methoxazole, trimethoprim and cycloserine.

AB - Identification of Clostridium botulinum is usually based on toxin detection of broth culture by mouse bioassay and requires 7 to 10 days to complete. Here, we describe an alternative in vitro procedure for direct identification of C. botulinum (types A and B) colonies which can be completed in 48 h. The method is based on toxigenicity of colonies demonstrable by enzyme immunoassay and resistance of C. botulinum to antimicrobial agents, sulpha-methoxazole, trimethoprim and cycloserine.

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KW - resistance

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Rapid identification of Clostridium botulinum colonies by in vitro toxicity and antimicrobial susceptibility testing

Abstract

Keywords

ASJC Scopus subject areas

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