Rapid identification of Clostridium botulinum colonies by in vitro toxicity and antimicrobial susceptibility testing

M. Dezfulian, J. G. Bartlett

Research output: Contribution to journalArticlepeer-review

Abstract

Identification of Clostridium botulinum is usually based on toxin detection of broth culture by mouse bioassay and requires 7 to 10 days to complete. Here, we describe an alternative in vitro procedure for direct identification of C. botulinum (types A and B) colonies which can be completed in 48 h. The method is based on toxigenicity of colonies demonstrable by enzyme immunoassay and resistance of C. botulinum to antimicrobial agents, sulpha-methoxazole, trimethoprim and cycloserine.

Original languageEnglish (US)
Pages (from-to)27-29
Number of pages3
JournalWorld Journal of Microbiology & Biotechnology
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • Clostridium botulinum
  • identification
  • immunoassay
  • resistance
  • toxigenicity

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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