TY - JOUR
T1 - Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis
AU - Yang, Samuel
AU - Ramachandran, Padmini
AU - Rothman, Richard
AU - Hsieh, Yu Hsiang
AU - Hardick, Andrew
AU - Won, Helen
AU - Kecojevic, Aleksandar
AU - Jackman, Joany
AU - Gaydos, Charlotte
PY - 2009/7
Y1 - 2009/7
N2 - A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.
AB - A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.
UR - http://www.scopus.com/inward/record.url?scp=67650087609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650087609&partnerID=8YFLogxK
U2 - 10.1128/JCM.00033-09
DO - 10.1128/JCM.00033-09
M3 - Article
C2 - 19458181
AN - SCOPUS:67650087609
SN - 0095-1137
VL - 47
SP - 2252
EP - 2255
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 7
ER -