Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis

Samuel Yang, Padmini Ramachandran, Richard Rothman, Yu Hsiang Hsieh, Andrew Hardick, Helen Won, Aleksandar Kecojevic, Joany Jackman, Charlotte Gaydos

Research output: Contribution to journalArticle

Abstract

A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.

Original languageEnglish (US)
Pages (from-to)2252-2255
Number of pages4
JournalJournal of clinical microbiology
Volume47
Issue number7
DOIs
StatePublished - Jul 1 2009

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ASJC Scopus subject areas

  • Microbiology (medical)

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