Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis

Helen Won, Richard Rothman, Padmini Ramachandran, Yu Hsiang Hsieh, Aleksandar Kecojevic, Karen C. Carroll, Deborah Aird, Charlotte Gaydos, Samuel Yang

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.

Original languageEnglish (US)
Pages (from-to)3410-3413
Number of pages4
JournalJournal of clinical microbiology
Volume48
Issue number9
DOIs
StatePublished - Sep 2010

ASJC Scopus subject areas

  • Microbiology (medical)

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