Rapid identification from rectal swabs of the clinically most relevant carbapenemase genes from gram-negative bacteria using the BD MAX Check-Points CPO Assay

Sergio García-Fernández, Patricia J. Simner, Gina Thomson, Matthew Faron, Ron Bosboom, Arjanne van Griethuijsen, María García-Castillo, Renee Harris, Nathan A. Ledeboer, Rafael Cantón, Kenneth S. Thomson

Research output: Contribution to journalArticlepeer-review

Abstract

We conducted an international multicentre evaluation to assess the clinical performance characteristics of the new multiplex PCR-based BD MAX Check-Points CPO assay to detect the 5 major carbapenemase families: KPC, VIM/IMP (tested simultaneously), NDM and OXA-48 compared to a reference method consisting of 2 culture methods (to improve recovery of CPO isolates from the rectal swabs), followed by carbapenem susceptibility testing and sequencing of target carbapenemase genes. Tests were performed from rectal swab specimens in ESwab collection and transport devices. Positive percent agreement (PPA) for BD MAX Check-Points CPO for KPC and OXA-48 were 88.2% (95% CI:72.6-96.7) and 96.2% (95% CI:80.4-99.9), respectively. Negative percent agreement was ≥99% for each gene. Insufficient samples (≤10) were positive for VIM/IMP or NDM tests to calculate meaningful PPA values. The BD MAX Check-Points CPO assay represents an accurate tool for rapid recognition of patients with rectal colonization by the most commonly encountered CPOs.

Original languageEnglish (US)
Article number115554
JournalDiagnostic Microbiology and Infectious Disease
Volume102
Issue number1
DOIs
StatePublished - Jan 2022

Keywords

  • BD MAX
  • Carbapenemase genes
  • Rapid detection
  • Rectal swabs

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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