Rapid generation of region specific probes by chromosome microdissection and their application

Paul S. Meltzer, Xin Yuan Guan, Ann Burgess, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

236 Scopus citations

Abstract

The strategy presented here to identify unequivocally cryptic chromosomal rearrangements has relevance to both prenatal and postnatal cytogenetic analysis as well as the analysis of tumour-associated chromosome rearrangements. Microdissection and in vitro amplification of specific chromosomal regions are performed, followed by labelling for fluorescent in situ hybridization (FISH) to normal metaphase chromosomes (Micro-FISH). Micro-FISH probes have been used successfully to determine the derivation of chromosome segments unidentifiable by standard chromosome banding analysis. Micro-FISH probes (created in less than 24 hours) now make it possible to identify explicitly the chromosome constitution of virtually all cytologically visible chromosome rearrangements.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalNature genetics
Volume1
Issue number1
DOIs
StatePublished - Apr 1992

ASJC Scopus subject areas

  • Genetics

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