Abstract
The strategy presented here to identify unequivocally cryptic chromosomal rearrangements has relevance to both prenatal and postnatal cytogenetic analysis as well as the analysis of tumour-associated chromosome rearrangements. Microdissection and in vitro amplification of specific chromosomal regions are performed, followed by labelling for fluorescent in situ hybridization (FISH) to normal metaphase chromosomes (Micro-FISH). Micro-FISH probes have been used successfully to determine the derivation of chromosome segments unidentifiable by standard chromosome banding analysis. Micro-FISH probes (created in less than 24 hours) now make it possible to identify explicitly the chromosome constitution of virtually all cytologically visible chromosome rearrangements.
Original language | English (US) |
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Pages (from-to) | 24-28 |
Number of pages | 5 |
Journal | Nature genetics |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - Apr 1992 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics