Rapid generation of region specific probes by chromosome microdissection and their application

Paul S. Meltzer, Xin Yuan Guan, Ann Burgess, Jeffrey M. Trent

Research output: Contribution to journalArticlepeer-review

Abstract

The strategy presented here to identify unequivocally cryptic chromosomal rearrangements has relevance to both prenatal and postnatal cytogenetic analysis as well as the analysis of tumour-associated chromosome rearrangements. Microdissection and in vitro amplification of specific chromosomal regions are performed, followed by labelling for fluorescent in situ hybridization (FISH) to normal metaphase chromosomes (Micro-FISH). Micro-FISH probes have been used successfully to determine the derivation of chromosome segments unidentifiable by standard chromosome banding analysis. Micro-FISH probes (created in less than 24 hours) now make it possible to identify explicitly the chromosome constitution of virtually all cytologically visible chromosome rearrangements.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalNature genetics
Volume1
Issue number1
DOIs
StatePublished - Apr 1992

ASJC Scopus subject areas

  • Genetics

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