Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures

M. Rahman, D. A. Sack, S. Mahmood, A. Hossain

Research output: Contribution to journalArticle

Abstract

A simple, rapid, and reliable method to detect Vibrio cholerae in fecal specimens would assist in the management of cases of severe diarrhea, especially since most such cases occur in areas with minimal laboratory facilities. A coagglutination test was used to detect V. cholerae antigen in bile-peptone broth incubated with feces. In the technique, Staphylococcus aureus Cowan I coated with anti-V. cholerae O1 antiserum was tested with cultures incubated for 4 h. When 165 specimens were tested, the sensitivity, specificity, and accuracy of the test, compared with standard culture methods, were 97, 99, and 98%, respectively. These promising results were better than those of dark-field microscopy using the same specimens, and the test was logistically easy to perform. The coagglutination test using enrichment broth culture of feces is a simple and rapid method which may be used to confirm a diagnosis of cholera.

Original languageEnglish (US)
Pages (from-to)2204-2206
Number of pages3
JournalJournal of Clinical Microbiology
Volume25
Issue number11
StatePublished - 1987
Externally publishedYes

Fingerprint

Cholera
Vibrio cholerae
Feces
Vibrio cholerae O1
Peptones
Case Management
Bile
Staphylococcus aureus
Immune Sera
Microscopy
Diarrhea
Antigens
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures. / Rahman, M.; Sack, D. A.; Mahmood, S.; Hossain, A.

In: Journal of Clinical Microbiology, Vol. 25, No. 11, 1987, p. 2204-2206.

Research output: Contribution to journalArticle

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