Rapid diagnosis of active tuberculosis by detecting antibodies from lymphocyte secretions

Rubhana Raqib, Jubayer Rahman, A. K.M. Kamaluddin, S. M.Mostafa Kamal, Fauzia A. Banu, Shakeel Ahmed, Zeaur Rahim, Pradip K. Bardhan, Jan Andersson, David A. Sack

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study, we investigated the tuberculosis (TB) diagnostic performance of an assay on the basis of detection of TB-specific antibodies from peripheral blood mononudear cells (PBMCs), to determine whether antibodies in lymphocyte secretions obtained from PBMCs would better reflect active disease than antibodies in serum. PBMCs from patients with and without TB cultured in various concentrations for different times were assessed. Immunoglobulin G (IgG) specific for antigen (bacille Calmette-Guérin [BCG] vaccine and purified protein derivative [PPD]) was measured in lymphocyte secretions. Patients with active TB had higher BCG- or PPD-specific IgG antibody responses than patients without TB or healthy subjects (P = .001). This method can be used as a quick diagnostic aid to facilitate rapid detection of TB cases.

Original languageEnglish (US)
Pages (from-to)364-370
Number of pages7
JournalJournal of Infectious Diseases
Volume188
Issue number3
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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