TY - JOUR
T1 - Rapid detection of herpes simplex virus in MRC-5 cells using low-speed centrifugation enhancement and immunoperoxidase staining 16 h post-inoculation
AU - Bonville, Cynthia A.
AU - Forbes, Betty A.
AU - Bartholoma, Nadine
AU - McMillan, Julia A.
AU - Weiner, Leonard B.
PY - 1987/12
Y1 - 1987/12
N2 - The performance of MRC-5 shell vial centrifugation-enhancement and direct immunoperoxidase staining was compared to the traditional WI38 tube cell culture for the detection of Herpes simplex virus on 123 clinical samples. The shell vial technology not only proved to be sensitive (100%) and specific (100%), but could offer up to 20% reduction in cost when compared to routine methods. In addition, a commercially prepared MRC-5 shell vial product proved to be superior with respect to cost, sensitivity, and reliability when compared to an in-house preparation.
AB - The performance of MRC-5 shell vial centrifugation-enhancement and direct immunoperoxidase staining was compared to the traditional WI38 tube cell culture for the detection of Herpes simplex virus on 123 clinical samples. The shell vial technology not only proved to be sensitive (100%) and specific (100%), but could offer up to 20% reduction in cost when compared to routine methods. In addition, a commercially prepared MRC-5 shell vial product proved to be superior with respect to cost, sensitivity, and reliability when compared to an in-house preparation.
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U2 - 10.1016/0732-8893(87)90057-5
DO - 10.1016/0732-8893(87)90057-5
M3 - Article
C2 - 2835202
AN - SCOPUS:0023568263
SN - 0732-8893
VL - 8
SP - 251
EP - 254
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -