Rapid Communication: Oxidative Stress Induces Apoptosis in Embryonic Cortical Neurons

Rajiv R. Ratan, Timothy H. Murphy, Jay M. Baraban

Research output: Contribution to journalArticle

Abstract

Abstract: Glutamate‐induced glutathione depletion in immature embryonic cortical neurons has been shown to lead to oxidative stress and cell death. We have used this in vitro model to investigate the mechanism(s) by which free radicals induce neuronal degeneration. We find that glutathione depletion leads to hypercondensation and fragmentation of chromatin into spherical or irregular shapes, a morphologic signature of apoptosis. These morphologic changes are accompanied by laddering of DNA into multiple oligonucleosomal fragments and can be prevented by the antioxidants idebenone and butylated hydroxyanisole. Cell death induced by glutathione depletion can also be prevented by inhibitors of macromolecular synthesis. Taken together, these observations suggest that oxidative stress can induce apoptosis in neurons.

Original languageEnglish (US)
Pages (from-to)376-379
Number of pages4
JournalJournal of Neurochemistry
Volume62
Issue number1
DOIs
StatePublished - Jan 1994

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Keywords

  • Apoptosis
  • Cortical neurons
  • Glutathione.
  • Oxidative stress

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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