TY - JOUR
T1 - Rapid chemically induced changes of PtdIns(4,5)P2 gate KCNQ ion channels
AU - Suh, Byung Chang
AU - Inoue, Takanari
AU - Meyer, Tobias
AU - Hille, Bertil
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - To resolve the controversy about messengers regulating KCNQ ion channels during phospholipase C-mediated suppression of current, we designed translocatable enzymes that quickly alter the phosphoinositide composition of the plasma membrane after application of a chemical cue. The KCNQ current falls rapidly to zero when phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P 2 or PI(4,5)P2] is depleted without changing Ca 2+, diacylglycerol, or inositol 1,4,5-trisphosphate. Current rises by 30% when PI(4,5)P2 is overproduced and does not change when phosphatidylinositol 3,4,5-trisphosphate is raised. Hence, the depletion of PI(4,5)P2 suffices to suppress current fully, and other second messengers are not needed. Our approach is ideally suited to study biological signaling networks involving membrane phosphoinositides.
AB - To resolve the controversy about messengers regulating KCNQ ion channels during phospholipase C-mediated suppression of current, we designed translocatable enzymes that quickly alter the phosphoinositide composition of the plasma membrane after application of a chemical cue. The KCNQ current falls rapidly to zero when phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P 2 or PI(4,5)P2] is depleted without changing Ca 2+, diacylglycerol, or inositol 1,4,5-trisphosphate. Current rises by 30% when PI(4,5)P2 is overproduced and does not change when phosphatidylinositol 3,4,5-trisphosphate is raised. Hence, the depletion of PI(4,5)P2 suffices to suppress current fully, and other second messengers are not needed. Our approach is ideally suited to study biological signaling networks involving membrane phosphoinositides.
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U2 - 10.1126/science.1131163
DO - 10.1126/science.1131163
M3 - Article
C2 - 16990515
AN - SCOPUS:33845310879
VL - 314
SP - 1454
EP - 1457
JO - Science
JF - Science
SN - 0036-8075
IS - 5804
ER -