Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines

Y. C. Lee; Jane R. Scocca; L. Muir

doi:10.1016/0003-2697(69)90070-0

Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines

Y. C. Lee, Jane R. Scocca, L. Muir

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A rapid method is described for the automatic determination of hexosamines in glycoproteins. Hexosamines are separated by cation-exchange chromatography and automatically determined by a neocuproine reducing sugar method. The method is highly reproducible and as little as 1 μg of glucosamine can be measured accurately.

Original language	English (US)
Pages (from-to)	559-566
Number of pages	8
Journal	Analytical biochemistry
Volume	27
Issue number	3
DOIs	https://doi.org/10.1016/0003-2697(69)90070-0
State	Published - Mar 1969
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(69)90070-0

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title = "Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines",

abstract = "A rapid method is described for the automatic determination of hexosamines in glycoproteins. Hexosamines are separated by cation-exchange chromatography and automatically determined by a neocuproine reducing sugar method. The method is highly reproducible and as little as 1 μg of glucosamine can be measured accurately.",

author = "Lee, {Y. C.} and Scocca, {Jane R.} and L. Muir",

note = "Funding Information: {\textquoteleft}This work was supported by Grant Health Service and a Faculty Research Contribution No. 544 from McCollum-Pratt sity, Baltimore, Maryland 21218. {\textquoteleft}U. S. Public Health Service acknowledged. “U. S. Public acknowledged. Funding Information: AM09970 from the United States Public Grant from the Johns Hopkins University. Institute, The Johns Hopkins Univer-",

year = "1969",

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doi = "10.1016/0003-2697(69)90070-0",

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T1 - Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines

AU - Lee, Y. C.

AU - Scocca, Jane R.

AU - Muir, L.

N1 - Funding Information: ‘This work was supported by Grant Health Service and a Faculty Research Contribution No. 544 from McCollum-Pratt sity, Baltimore, Maryland 21218. ‘U. S. Public Health Service acknowledged. “U. S. Public acknowledged. Funding Information: AM09970 from the United States Public Grant from the Johns Hopkins University. Institute, The Johns Hopkins Univer-

PY - 1969/3

Y1 - 1969/3

N2 - A rapid method is described for the automatic determination of hexosamines in glycoproteins. Hexosamines are separated by cation-exchange chromatography and automatically determined by a neocuproine reducing sugar method. The method is highly reproducible and as little as 1 μg of glucosamine can be measured accurately.

AB - A rapid method is described for the automatic determination of hexosamines in glycoproteins. Hexosamines are separated by cation-exchange chromatography and automatically determined by a neocuproine reducing sugar method. The method is highly reproducible and as little as 1 μg of glucosamine can be measured accurately.

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Rapid automatic analysis of sugar components in glycoproteins. I. Hexosamines

Abstract

ASJC Scopus subject areas

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