Rapid assay of cocaine, opiates and metabolites by gas chromatography-mass spectrometry

Edward J. Cone, William D. Darwin

Research output: Contribution to journalReview articlepeer-review

32 Scopus citations


The simultaneous assay of cocaine, opiates and metabolites in small biological samples continues to be a difficult task. This report focuses upon tabulation of important techniques (extraction, derivatization, chromatographic conditions, detection mode, data acquisition) reported over the last decade that were used in the development of assays for these analytes. The most prevalent procedures for extraction of cocaine, opiates and metabolites were liquid-liquid and solid-phase extraction isolation methods. Following extraction analytes were derivatized and analyzed by gas chromatography-mass spectrometry. The technique most often used for chromatographic separation was fused-silica capillary column gas chromatography. Detection generally was performed by selected ion monitoring in the positive-ion electron-impact ionization mode, although full-scan acquisition and positive- and negative-ion chemical ionization methods have been used. It was apparent from the review that there is a continuing need for greater sensitivity and selectivity in the assay of highly potent opiates and for cocaine and metabolites.

Original languageEnglish (US)
Pages (from-to)43-61
Number of pages19
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number1-2
StatePublished - Sep 16 1992

ASJC Scopus subject areas

  • Chemistry(all)


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