TY - JOUR
T1 - Rapid anterograde axonal transport of the cellular prion glycoprotein in the peripheral and central nervous systems
AU - Borchelt, David R.
AU - Koliatsos, Vassilis E.
AU - Guarnieri, Michael
AU - Pardo, Carlos A.
AU - Sisodia, Sangram S.
AU - Price, Donald L.
PY - 1994/5/20
Y1 - 1994/5/20
N2 - In prion diseases, the cellular prion protein (PrP(C)), abundant in neurons, is converted posttranslationally into an amyloid-forming scrapie prion protein (PrP(Sc)), which accumulates in white matter tracts and nerve terminals. The trafficking of PrP(C) in neurons was investigated in vivo by injecting [35S]methionine into the L4 and L5 dorsal root ganglia and the entorhinal cortices of adult rats and by tracing the movement of radiolabeled PrP(C). In both paradigms, labeled 33-35-kDa PrP(C) was transported, within 4 h, to distal axons and nerve terminals cofractionating with proteins in the fast component. Future studies using these methods may allow us to determine whether PrP(C) is converted into PrP(Sc) during axonal transport and whether PrP(Sc) is transported in animals with prion diseases.
AB - In prion diseases, the cellular prion protein (PrP(C)), abundant in neurons, is converted posttranslationally into an amyloid-forming scrapie prion protein (PrP(Sc)), which accumulates in white matter tracts and nerve terminals. The trafficking of PrP(C) in neurons was investigated in vivo by injecting [35S]methionine into the L4 and L5 dorsal root ganglia and the entorhinal cortices of adult rats and by tracing the movement of radiolabeled PrP(C). In both paradigms, labeled 33-35-kDa PrP(C) was transported, within 4 h, to distal axons and nerve terminals cofractionating with proteins in the fast component. Future studies using these methods may allow us to determine whether PrP(C) is converted into PrP(Sc) during axonal transport and whether PrP(Sc) is transported in animals with prion diseases.
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M3 - Article
C2 - 7514179
AN - SCOPUS:0028356488
VL - 269
SP - 14711
EP - 14714
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 20
ER -