Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling

Askar M. Akimzhanov, Darren Boehning, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Palmitoylation is the posttranslational modification of proteins with a 16-carbon fatty acid chain through a labile thioester bond. The reversibility of protein palmitoylation and its profound effect on protein function suggest that this modification could play an important role as an intracellular signaling mechanism. Evidence that palmitoylation of proteins occurs with the kinetics required for signal transduction is not clear, however. Here we show that engagement of the Fas receptor by its ligand leads to an extremely rapid and transient increase in palmitoylation levels of the tyrosine kinase Lck. Lck palmitoylation kinetics are consistent with the activation of downstream signaling proteins, such as Zap70 and PLC-γ1. Inhibiting Lck palmitoylation not only disrupts proximal Fas signaling events, but also renders cells resistant to Fas-mediated apoptosis. Knockdown of the palmitoyl acyl transferase DHHC21 eliminates activation of Lck and downstream signaling after Fas receptor stimulation. Our findings demonstrate highly dynamic Lck palmitoylation kinetics that are essential for signaling downstream of the Fas receptor.

Original languageEnglish (US)
Pages (from-to)11876-11880
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number38
DOIs
StatePublished - Sep 22 2015

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Lipoylation
Protein-Tyrosine Kinases
CD95 Antigens
Proteins
Post Translational Protein Processing
Transferases
Signal Transduction
Fatty Acids
Carbon
Apoptosis
Ligands

Keywords

  • Apoptosis
  • Calcium
  • Fas
  • Lck
  • Protein palmitoylation

ASJC Scopus subject areas

  • General

Cite this

Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling. / Akimzhanov, Askar M.; Boehning, Darren; Snyder, Solomon H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 38, 22.09.2015, p. 11876-11880.

Research output: Contribution to journalArticle

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