TY - JOUR
T1 - Rapid and specific conversion of precursor interleukin 1β (IL-1β) to an active IL-1 species by human mast cell chymase
AU - Mizutani, Hitoshi
AU - Schechter, Norman
AU - Lazarus, Gerald
AU - Black, Roy A.
AU - Kupper, Thomas S.
PY - 1991/10/1
Y1 - 1991/10/1
N2 - Secretory granules of human dermal mast cells contain a chymotrypsin-like serine proteinase called chymase. In this study, we demonstrate that the inactive cytokine, 31 kD interleukin 1β (IL-1β), can be converted rapidly to an 18 kD biologically active species by human mast cell chymase. The product formed is three amino acids longer at the amino terminus than the mature IL-1β produced by peripheral blood mononuclear cells and has comparable biological activity. Because chymase is a secretory granule constituent, it is likely to be released into the surrounding tissue when mast cells degranulate. It is also known that non-bone marrow derived cells resident in skin (keratinocytes, fibroblasts) produce but do not process 31 kD IL-1β. In this context, chymase may be a potent activator of locally produced 31 kD IL-1β. Mast cells lie in close apposition to blood vessels in dermis; therefore, chymase mediated conversion of 31 kD IL-1β might be expected to have a critical role in the initiation of the inflammatory response in skin.
AB - Secretory granules of human dermal mast cells contain a chymotrypsin-like serine proteinase called chymase. In this study, we demonstrate that the inactive cytokine, 31 kD interleukin 1β (IL-1β), can be converted rapidly to an 18 kD biologically active species by human mast cell chymase. The product formed is three amino acids longer at the amino terminus than the mature IL-1β produced by peripheral blood mononuclear cells and has comparable biological activity. Because chymase is a secretory granule constituent, it is likely to be released into the surrounding tissue when mast cells degranulate. It is also known that non-bone marrow derived cells resident in skin (keratinocytes, fibroblasts) produce but do not process 31 kD IL-1β. In this context, chymase may be a potent activator of locally produced 31 kD IL-1β. Mast cells lie in close apposition to blood vessels in dermis; therefore, chymase mediated conversion of 31 kD IL-1β might be expected to have a critical role in the initiation of the inflammatory response in skin.
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M3 - Article
C2 - 1919436
AN - SCOPUS:0026040529
SN - 0022-1007
VL - 174
SP - 821
EP - 825
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 4
ER -