Rapid and sensitive detection of viral nucleic acids using silicon microchips

Laura Powell, Rodrigo Sergio Wiederkehr, Paige Damascus, Maarten Fauvart, Federico Buja, Tim Stakenborg, Stuart Campbell Ray, Paolo Fiorini, William Osburn

Research output: Contribution to journalArticle

Abstract

Clinical laboratory-based nucleic acid amplification tests (NAT) play an important role in diagnosing viral infections. However, laboratory infrastructure requirements and their failure to diagnose at the point-of-need (PON) limit their clinical utility in both resource-rich and -limited clinical settings. The development of fast and sensitive PON viral NAT may overcome these limitations. The scalability of silicon microchip manufacturing combined with advances in silicon microfluidics present an opportunity for development of rapid and sensitive PON NAT on silicon microchips. In the present study, we present rapid and sensitive NAT for a number of RNA and DNA viruses on the same silicon microchip platform. We first developed sensitive (4 copies per reaction) one-step RT-qPCR and qPCR assays detecting HCV, HIV, Zika, HPV 16, and HPV 18 on a benchtop real-time PCR instrument. A silicon microchip was designed with an etched 1.3 μL meandering microreactor, integrated aluminum heaters, thermal insulation trenches and microfluidic channels; this chip was used in all on-chip experiments. Melting curve analysis confirmed precise and localized heating of the microreactor. Following minimal optimization of reaction conditions, the bench-scale assays were successfully transferred to 1.3 μL silicon microreactors with reaction times of 25 min with no reduction in sensitivity, reproducibility, or reaction efficiencies. Taken together, these results demonstrate that rapid and sensitive detection of multiple viruses on the same silicon microchip platform is feasible. Further development of this technology, coupled with silicon microchip-based nucleic acid extraction solutions, could potentially shift viral nucleic acid detection and diagnosis from centralized clinical laboratories to the PON.

Original languageEnglish (US)
Pages (from-to)2596-2603
Number of pages8
JournalAnalyst
Volume143
Issue number11
DOIs
StatePublished - Jun 7 2018

Fingerprint

Nucleic acids
nucleic acid
Silicon
Nucleic Acids
silicon
Nucleic Acid Amplification Techniques
Amplification
amplification
Clinical laboratories
Microfluidics
Viruses
Assays
virus
assay
Human papillomavirus 18
DNA Viruses
Human papillomavirus 16
detection
RNA Viruses
Thermal insulation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Powell, L., Wiederkehr, R. S., Damascus, P., Fauvart, M., Buja, F., Stakenborg, T., ... Osburn, W. (2018). Rapid and sensitive detection of viral nucleic acids using silicon microchips. Analyst, 143(11), 2596-2603. https://doi.org/10.1039/c8an00552d

Rapid and sensitive detection of viral nucleic acids using silicon microchips. / Powell, Laura; Wiederkehr, Rodrigo Sergio; Damascus, Paige; Fauvart, Maarten; Buja, Federico; Stakenborg, Tim; Ray, Stuart Campbell; Fiorini, Paolo; Osburn, William.

In: Analyst, Vol. 143, No. 11, 07.06.2018, p. 2596-2603.

Research output: Contribution to journalArticle

Powell, L, Wiederkehr, RS, Damascus, P, Fauvart, M, Buja, F, Stakenborg, T, Ray, SC, Fiorini, P & Osburn, W 2018, 'Rapid and sensitive detection of viral nucleic acids using silicon microchips', Analyst, vol. 143, no. 11, pp. 2596-2603. https://doi.org/10.1039/c8an00552d
Powell L, Wiederkehr RS, Damascus P, Fauvart M, Buja F, Stakenborg T et al. Rapid and sensitive detection of viral nucleic acids using silicon microchips. Analyst. 2018 Jun 7;143(11):2596-2603. https://doi.org/10.1039/c8an00552d
Powell, Laura ; Wiederkehr, Rodrigo Sergio ; Damascus, Paige ; Fauvart, Maarten ; Buja, Federico ; Stakenborg, Tim ; Ray, Stuart Campbell ; Fiorini, Paolo ; Osburn, William. / Rapid and sensitive detection of viral nucleic acids using silicon microchips. In: Analyst. 2018 ; Vol. 143, No. 11. pp. 2596-2603.
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