Rapid and dynamic alterations of gene expression profiles of adult porcine bone marrow-derived stem cell in response to hypoxia

Suna Wang, Yifu Zhou, Caleb N. Seavey, Avneesh Singh, Xiuli Xu, Timothy Hunt, Robert F. Hoyt, Keith A. Horvath

Research output: Contribution to journalArticle

Abstract

This study sought to identify the gene expression patterns of porcine bone marrow-derived MSC in response to hypoxia and to investigate novel specific hypoxic targets that may have a role in determining MSC proliferation/survival and differentiation. MSC from 15 animals were incubated in 1% oxygen and 8% carbon dioxide for 6, 12, and 24 h. RNA samples were isolated and assayed with Affymetrix porcine arrays and quantitative reverse-transcription PCR. Significant gene expression levels among the four groups of normoxia, 6-, 12-, and 24-h hypoxia were identified. The pattern in the 12-h hypoxia group was similar to that of the 24-h group. Of 23,924 probes, 377 and 210 genes were regulated in the 6- and 24-h hypoxia groups, respectively. Functional classification of the hypoxic regulated genes was mainly clustered in cell proliferation and response to stress. However, the major upregulated genes in the 6-h group were activated in cell cycle phases; the genes in the 24-h hypoxia were evenly separated into cell differentiation, apoptosis, and cellular metabolic processes. Twenty-eight genes were upregulated in all hypoxia groups; these genes are considered as hypoxic targets. Our results identified a genome-wide hypoxia-induced gene expression pattern in porcine MSC. This study provides a global view of molecular events in the cells during exposure to hypoxia and revealed a set of novel candidate hypoxic targets.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalStem Cell Research
Volume4
Issue number2
DOIs
StatePublished - Mar 2010
Externally publishedYes

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Transcriptome
Swine
Stem Cells
Bone Marrow
Genes
Gene Expression
Hypoxia
Carbon Dioxide
Reverse Transcription
Cell Differentiation
Cell Cycle
Cell Proliferation
Genome
RNA
Apoptosis
Oxygen
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Rapid and dynamic alterations of gene expression profiles of adult porcine bone marrow-derived stem cell in response to hypoxia. / Wang, Suna; Zhou, Yifu; Seavey, Caleb N.; Singh, Avneesh; Xu, Xiuli; Hunt, Timothy; Hoyt, Robert F.; Horvath, Keith A.

In: Stem Cell Research, Vol. 4, No. 2, 03.2010, p. 117-128.

Research output: Contribution to journalArticle

Wang, Suna ; Zhou, Yifu ; Seavey, Caleb N. ; Singh, Avneesh ; Xu, Xiuli ; Hunt, Timothy ; Hoyt, Robert F. ; Horvath, Keith A. / Rapid and dynamic alterations of gene expression profiles of adult porcine bone marrow-derived stem cell in response to hypoxia. In: Stem Cell Research. 2010 ; Vol. 4, No. 2. pp. 117-128.
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