Rapid adaptive optical recovery of optimal resolution over large volumes

Kai Wang, Daniel E. Milkie, Ankur Saxena, Peter Engerer, Thomas Misgeld, Marianne E. Bronner, Jeff Mumm, Eric Betzig

Research output: Contribution to journalReview articlepeer-review

Abstract

Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

Original languageEnglish (US)
Pages (from-to)625-628
Number of pages4
JournalNature Methods
Volume11
Issue number6
DOIs
StatePublished - Jun 2014

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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