Rapid adaptive optical recovery of optimal resolution over large volumes

Kai Wang, Daniel E. Milkie, Ankur Saxena, Peter Engerer, Thomas Misgeld, Marianne E. Bronner, Jeff Mumm, Eric Betzig

Research output: Contribution to journalReview article

Abstract

Using a descanned, laser-induced guide star and direct wavefront sensing, we demonstrate adaptive correction of complex optical aberrations at high numerical aperture (NA) and a 14-ms update rate. This correction permits us to compensate for the rapid spatial variation in aberration often encountered in biological specimens and to recover diffraction-limited imaging over large volumes (>240 mm per side). We applied this to image fine neuronal processes and subcellular dynamics within the zebrafish brain.

Original languageEnglish (US)
Pages (from-to)625-628
Number of pages4
JournalNature Methods
Volume11
Issue number6
DOIs
StatePublished - Jun 2014

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Rapid adaptive optical recovery of optimal resolution over large volumes'. Together they form a unique fingerprint.

  • Cite this

    Wang, K., Milkie, D. E., Saxena, A., Engerer, P., Misgeld, T., Bronner, M. E., Mumm, J., & Betzig, E. (2014). Rapid adaptive optical recovery of optimal resolution over large volumes. Nature Methods, 11(6), 625-628. https://doi.org/10.1038/nmeth.2925