Randomly amplified polymorphic DNA as a tool for genotoxicity: An assessment

Sanjeev Noel, Srikanta Kumar Rath

Research output: Contribution to journalArticle

Abstract

Novel short-term assays are required to substantiate the battery of assessment methods for evaluating the genotoxicity of candidate drugs. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish genotoxic effect of a known genotoxicant, ie, ethyl methanesulfonate (EMS) in Swiss mice (Mus musculus). Based on the RAPD profiles, genetic damages were detected in EMS-exposed animals, suggesting its usefulness in scanning whole genome for assessing the genotoxic effects of candidate drugs. The profiles were generated using genomic DNA, isolated from liver prior to treatment and from liver, bone marrow and blood after treatment of the genotoxicant. Measurable differences indicative of genetic damages were observed when the pre- and post-treatment profiles were compared. This suggests that RAPD analysis may be useful for assessing the pre-clinical genotoxic effects of candidate drugs.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalToxicology and Industrial Health
Volume22
Issue number6
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • RAPD analysis
  • Swiss mice
  • ethyl methanesulfonate
  • genotoxicity

ASJC Scopus subject areas

  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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