RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor

Scott Powers, Susan Michaelis, Daniel Broek, Anna A. Sonia Santa, Jeffrey Field, Ira Herskowitz, Michael Wigler

Research output: Contribution to journalArticlepeer-review

Abstract

We have identified a gene (SUPH) of S. cerevisiae that is required for both RAS function and mating by cells of a mating type. supH is allelic to ste16, a gene required for the production of the mating pheromone a-factor. Both RAS and a-factor coding sequences terminate with the potential acyltransferase recognition sequence Cys-A-A-X, where A is an aliphatic amino acid. Mutations in SUPH-STE16 prevent the membrane localization and maturation of RAS protein, as well as the fatty acid acylation of it and other membrane proteins. We propose the designation RAM (RAS protein and a-factor maturation function) for SUPH and STE16. RAM may encode an enzyme responsible for the modification and membrane localization of proteins with this C-terminal sequence.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalCell
Volume47
Issue number3
DOIs
StatePublished - Nov 7 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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