Rage gene promoter polymorphisms and diabetic retinopathy in a clinic-based population from South India

S. Ramprasad, V. Radha, Rasika Mathias, P. P. Majumder, M. R S Rao, M. Rema

Research output: Contribution to journalArticle

Abstract

Purpose: The main objective of this study was to evaluate if the -429T/C, -374T/A and 63bp deletion polymorphisms in the RAGE gene are associated with diabetic retinopathy (DR) among Type 2 diabetic subjects in a clinic-based population from South India. Methods: We screened 149 normal glucose tolerant subjects (NGT), 189 Type 2 diabetes subjects without retinopathy (DM) and 190 subjects with DR for these polymorphisms using the PCR-RFLP method. DR was diagnosed by grading color fundus photography. Logistic regression models were used to evaluate the association of individual polymorphisms with DR. Expectation-maximization algorithms were implemented in haplotype tests of association to examine the combined effects of -429T/C and -374T/A polymorphisms on DR. Results: The allelic frequencies of -429T are 0.83 in NGT, 0.84 in DM and 0.85 in DR subjects, and that of -374T are 0.93 in NGT, 0.92 in DM and 0.88 in DR subjects. The -374 polymorphism was found to be associated with non-proliferative retinopathy when this subgroup was compared to the DM group (OR=1.814, 95% CI=1.005-3.273). However, this association was not obvious when both the subphenotypes of DR (the nonproliferative and proliferative DR groups) were studied jointly. We found no evidence for associations between the -429T/C polymorphism and the DR phenotype. Finally, extension to a 2-SNP haplotype did not reveal any significant statistical difference between the groups (P=0.668). Conclusion: In this study, we found a modest association with the -374T/ A polymorphism in the nonproliferative DR subgroup.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalEye (London, England)
Volume21
Issue number3
DOIs
StatePublished - Mar 2007
Externally publishedYes

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Rage
Diabetic Retinopathy
India
Population
Genes
Glucose
Haplotypes
Logistic Models
Photography
Restriction Fragment Length Polymorphisms
Type 2 Diabetes Mellitus
Single Nucleotide Polymorphism
Color

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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Rage gene promoter polymorphisms and diabetic retinopathy in a clinic-based population from South India. / Ramprasad, S.; Radha, V.; Mathias, Rasika; Majumder, P. P.; Rao, M. R S; Rema, M.

In: Eye (London, England), Vol. 21, No. 3, 03.2007, p. 395-401.

Research output: Contribution to journalArticle

Ramprasad, S. ; Radha, V. ; Mathias, Rasika ; Majumder, P. P. ; Rao, M. R S ; Rema, M. / Rage gene promoter polymorphisms and diabetic retinopathy in a clinic-based population from South India. In: Eye (London, England). 2007 ; Vol. 21, No. 3. pp. 395-401.
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abstract = "Purpose: The main objective of this study was to evaluate if the -429T/C, -374T/A and 63bp deletion polymorphisms in the RAGE gene are associated with diabetic retinopathy (DR) among Type 2 diabetic subjects in a clinic-based population from South India. Methods: We screened 149 normal glucose tolerant subjects (NGT), 189 Type 2 diabetes subjects without retinopathy (DM) and 190 subjects with DR for these polymorphisms using the PCR-RFLP method. DR was diagnosed by grading color fundus photography. Logistic regression models were used to evaluate the association of individual polymorphisms with DR. Expectation-maximization algorithms were implemented in haplotype tests of association to examine the combined effects of -429T/C and -374T/A polymorphisms on DR. Results: The allelic frequencies of -429T are 0.83 in NGT, 0.84 in DM and 0.85 in DR subjects, and that of -374T are 0.93 in NGT, 0.92 in DM and 0.88 in DR subjects. The -374 polymorphism was found to be associated with non-proliferative retinopathy when this subgroup was compared to the DM group (OR=1.814, 95{\%} CI=1.005-3.273). However, this association was not obvious when both the subphenotypes of DR (the nonproliferative and proliferative DR groups) were studied jointly. We found no evidence for associations between the -429T/C polymorphism and the DR phenotype. Finally, extension to a 2-SNP haplotype did not reveal any significant statistical difference between the groups (P=0.668). Conclusion: In this study, we found a modest association with the -374T/ A polymorphism in the nonproliferative DR subgroup.",
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AU - Rao, M. R S

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