Radiosensitization mechanism of riboflavin in vitro

Guanshu Liu, Changyuan Lu, Side Yao, Fang Zhao, Yu Li, Xiangshun Meng, Jianguo Gao, Jianming Cai, Liming Zhang, Zhilong Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Riboflavin, suggested to be a radiosensitizer, was studied in murine thymocytes and human hepatoma L02 cell line in vitro with MTT method and fluorescence microscopy. When the murine thymocytes treated with 5-400 μmol/L riboflavin were irradiated by 5 Gy 60Co γ ionizing radiation, the low concentration groups, i.e. treated with 5-50 μmol/L riboflavin, showed a different surviving fractions-time relating correlation compared with the high concentration groups, i.e. treated with 100-400 μmol/L riboflavin. The former had a high survival level at the end of irradiation, but which, after 4-h incubation, decreased rapidly to a low level. On the contrary, the high concentration groups showed a low survival level at the end of irradiation, and a poor correlation was found between the surviving fraction and the incubation time, after 4 h a little difference was observed. The results of fluorescence microscopy indicated that under low concentration conditions, the riboflavin localized mainly in nucleus (both perinuclear area and inside of nuclear membrane), while under high concentration conditions, intensive riboflavin also localized around cytoplasmic membranes. Thus we can conclude: the riboflavin had radiosensitivity effect on DNA under low concentration conditions, and enhanced the damage to cytoplasmic membrane under high concentration conditions. Also the most effective concentration of riboflavin can be evaluated to be approximate 100 μmol/L.

Original languageEnglish (US)
Pages (from-to)344-352
Number of pages9
JournalScience in China, Series C: Life Sciences
Volume45
Issue number4
DOIs
StatePublished - 2002

Keywords

  • Fluorescence microscopy
  • L02 cell
  • MTT method
  • Murine thymocyte
  • Radiosensitization
  • Riboflavin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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