Abstract
Purpose: The purpose of this paper is to study the association between RGD binding kinetics and αvβ3 integrin receptor density in the complex tumor milieu. Procedures: We assessed αvβ3 in vitro and by 68Ga-DOTA- [c(RGDfK)]2 positron emission tomography (PET) in tumors with varying αvβ3. Results: Intrinsic α vβ3 expression decreased in the order of M21⋙MDA-MB-231>M21L in cells. Tumor volume of distribution by PET, V T, was significantly higher in M21 compared to isogenic M21L tumors (0.40±0.01 versus 0.25±0.02; p<0.01) despite similar microvessel density (MVD) likely due to higher αvβ 3. VT for MDA-MB-231 (0.40±0.04) was comparable to M21 despite lower αvβ3 but in keeping with the higher MVD, suggesting superior tracer distribution. Conclusions: This study demonstrates that radioligand binding kinetics of PET data can be used to discriminate tumors with different αvβ3 integrin expression-a key component of the angiogenesis phenotype - in vivo.
Original language | English (US) |
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Pages (from-to) | 558-566 |
Number of pages | 9 |
Journal | Molecular Imaging and Biology |
Volume | 16 |
Issue number | 4 |
DOIs | |
State | Published - Aug 2014 |
Externally published | Yes |
Keywords
- Integrin
- Kinetic modeling
- Positron emission tomography
- RGD peptide
- Vasculature
ASJC Scopus subject areas
- Oncology
- Radiology Nuclear Medicine and imaging
- Cancer Research